WT_1蛋白在肾母细胞瘤与肾发育过程中的表达  

Expression of WT_1 gene in nephroblastoma and developing kidneys

作　　者：王金根[1] 高解春[1] 陈莲[1] 

机构地区：[1]上海医科大学儿科医院外科,200032

出　　处：《中华小儿外科杂志》2000年第3期156-158,共3页Chinese Journal of Pediatric Surgery

摘　　要：目的　探讨WT1基因在肾发育和肾母细胞瘤发生中的作用。方法　用免疫组化方法 ,研究 2 7例不同胎龄肾脏和 17例肾母细胞瘤组织中WT1蛋白的表达。结果　WT1蛋白在小胎龄肾组织中未成熟的肾小球细胞核表达阳性率为 5 7.1% ,在大胎龄肾组织中肾小管细胞浆表达阳性率为46 .2 % ,在肾母细胞瘤的胚基上皮成分中表达阳性率为 41.2 % ,间质成分无表达。结论　WT1基因对胎肾的早期分化和后期发育成熟都有重要作用 ;WT1表达异常与肾母细胞瘤的发生和组织分化有关。Objective To evaluate the role of WT 1 gene in the development of human kidney and nephroblastoma. Methods Expression of WT 1 protein in the formalin-fixed and paraffin-embedded kidney specimens of 27 fetus at various stages of gestation and 17 nephroblastomas were assessed immunohistochemically. Results Nuclear expression of WT 1 protein was observed on 57.1% of early fetal glomeruli. Cytoplasmic expression was detected in 46.2% late fetal renal tubules. Nuclear expression was found in the blastemal and epithelial regions in 41.2% of nephroblastoma. Conclusions WT 1 gene may play an important role in early differentiation of human kidney as well as in later development. The abnormal expression of WT 1 may be related to the development of nephroblastoma.

关 键 词：肾母细胞瘤 免疫组织化学 肾发育 WT1蛋白 

分 类 号：R737.11[医药卫生—肿瘤]