蛋白激酶CK2α在人甲状腺鳞癌细胞SW579中的表达和活性  

Expression and its significance of protein kinase CK2α in human thyroid carcinoma SW579 cells

作　　者：谷迪西[1] 刘超[1] 刘洋[1] 肖建英[1] 

机构地区：[1]辽宁医学院生物化学与分子生物学教研室,辽宁省锦州121001

出　　处：《中国医师杂志》2013年第5期595-598,共4页Journal of Chinese Physician

基　　金：辽宁省教育厅重点实验室项目（LS2010101）

摘　　要：目的研究蛋白激酶CK2α在人甲状腺鳞癌细胞SW579中的表达和活性，探讨其与甲状腺癌细胞之间的关系。方法体外培养SW579和Hacat细胞，应用逆转录-聚合酶链反应（RT-PCR）和Westernblotting技术分别检测蛋白激酶CK2α mRNA和蛋白在SW579细胞和Hacat细胞中的表达；应用放射自显影法检测细胞内蛋白激酶CK2的活性。结果蛋白激酶CK2α mRNA和蛋白在SW579细胞中高表达，在Hacat细胞中低表达（mRNA：0．92±0．01vs0．52±0．02，t=19．24，P〈0．01；蛋白：0．98±0．01vs0．37±0．02，t=24．14，P〈0．01）；SW579细胞中CK2的活性高于Hacat细胞（4911±151．30vs1999±87．90，t=16．64，P〈0．01）。结论蛋白激酶CK2α过表达可能与甲状腺癌的发生和发展有关。Objective To investigate the expression of protein kinase CK2α in thyroid carcinoma SW579 cells and its significance. Methods SW579 cells and Hacat cells were cultured in vitro and the ex- pression levels of protein kinase CK2α mRNA and protein in SW579 cells and Hacat cells were measured by reverse transcription-polymerase chain reaction （RT-PCR） and Western blotting. Moreover, CK2 activity was also measured. Results It was showed that the mRNA （0. 92 ±0. 01vs 0. 52±0. 02, t =19.24,P 〈0.01） and protein（0. 98 ±0. 01 vs 0. 37 ±0. 02, t =24. 14, P 〈0. 01 ） expression of protein kinase CK2αwere sig- nificantly stronger in SW579 cells relative to Hacat cells. Conclusions Formation and development of thyroid carcinoma may be in connection with the overexpression of protein kinase CK2α.

关 键 词：蛋白激酶类 代谢 甲状腺肿瘤 病理学 癌 鳞状细胞 病理学 肿瘤细胞 培养的 

分 类 号：R736.1[医药卫生—肿瘤]