壳聚糖-丝素蛋白-磷酸三钙支架的力学性能及异位成骨能力的研究  

Osteogenetic potentials and mechanical properties of the periodontal tissue engineering scaffold made of chitosan-silk fibroin-tricalcium

作　　者：张蕊[1] 张芳[2] 赵彬[3] 柏国艳[1] 

机构地区：[1]山西医科大学口腔医学系,太原030001 [2]山西医科大学口腔医学系基础教研室,太原030001 [3]山西医科大学口腔医学系修复教研室,太原030001

出　　处：《中国药物与临床》2013年第6期695-698,共4页Chinese Remedies & Clinics

基　　金：山西省科技攻关项目(20090311057-9)

摘　　要：目的对壳聚糖-丝素蛋白支架(CS-SF)与壳聚糖-丝素蛋白-磷酸三钙支架(CS-SF-TCP)的力学性能及早期体内成骨能力进行实验研究,以寻求更适合牙周组织工程的支架材料。方法采用二次冻干技术制备2种支架,用Instron5544拉伸实验机对该2种支架的压缩模量进行测试,并将2种支架与矿化诱导后的骨髓基质细胞(BMSCs)复合培养3d后无菌条件下移植入裸鼠背部皮下,4周时取出标本行大体观察、苏木精-伊红染色、Ⅰ型胶原免疫组织化学染色,观察2种支架的体内成骨能力。结果力学性能检测结果显示:CS-SF组与CS-SF-TCP组的抗压强度分别为(0.44±0.15)MPa、(1.44±0.96)MPa。CS-SF-TCP组高于CS-SF组(P<0.05)。早期体内成骨能力结果:肉眼观察取出的植入物均有薄膜包绕。HE染色可见CS-SF+BMSCs与CS-SF-TCP+BMSCs组可见支架中心长入大量的细胞并有胶原样物质生成。免疫组织化学见2组均有不同程度的Ⅰ型胶原阳性表达,CS-SF-TCP+BMSCs组表达要高于CS-SF+BMSCs组(P<0.05)。结论与CS-SF支架相比,CS-SF-TCP支架有更好的力学性能及诱导BMSCs形成新生的骨组织的能力,更适合于牙周组织工程的支架。Objective To find a better scaffold material for periodontal tissue engineering by comparing the mechanical properties and osteogenic potentials of the chitosan-silk fibroin （CS-SF） scaffold versus the chitosan-silk fibroin-tricalcium （CS-SF-TCP） scaffold. Methods The 2 sorts of scaffolds were prepared by freezing and lyophilizing techniques. The modulus of compressibility in the 2 scaffolds was determined by a tensile testing machine （INSTRON 5544）. Mouse bone marrow stromal cells （BMSCs） were incubated in the 2 scaffolds for 3 days before the complexes were implanted aseptically into the subcutaneous back of nude mice. Four weeks after implantation, specimens were harvested for gross observation, hematoxylin-eosin staining and immunohistochemical staining of type Ⅰ collagen to assess the in vivo osteogenic potentials of the 2 scaffolds. Results The compressive strength of the CS-SF-TCP scaffold [（1.44±0.96） MPa] was significantly higher than that of the CS-SF scaffold [（0.44±0.15） MPa] （P〈0.05）. All the implants were covered by a thick membrane by gross observation. HE staining showed both CS-SF＋BMSCs and CS- SF-TCP＋BMSCs complexes had massive cellular proliferation and collagen synthesis in the pores of the 2 scaffolds. Expressions of type Ⅰ collagen were observed in the 2 complexes but the expression in the CS-SF-TCP＋BMSCs was significantly higher than in the CS-SF＋BMSCs （P〈0.05）. Conclusion The CS-SF-TCP scaffold is more suitable for periodontal tissue engineering because it possesses better biomechanical performance and a greater osteogenic potential than the CS-SF scaffold.

关 键 词：牙周组织 丝素蛋白 组织工程支架 壳聚糖 裸鼠 磷酸三钙 

分 类 号：R782[医药卫生—口腔医学]