壳聚糖固定化酶一步纯化抑肽酶研究  

A new method of preparation of high-Purity aprotininum by chemicol modified trypsin of chitosan

作　　者：宋扬[1] 侯司 赵辉[1] 袁克文 石华峰 汪姗霖 

机构地区：[1]安徽省生物研究所,合肥230031 [2]阜阳生化制药厂,阜阳236090

出　　处：《生物学杂志》2000年第1期18-21,共4页Journal of Biology

基　　金：安徽省"九五"科技攻关项目!(961 30 1 4 )

摘　　要：采用化学改性与修饰微珠壳聚糖为栽体,共价法偶联牛胰蛋白酶,制成抑肽酶亲和吸附剂,单位活力5190KIU/g(湿),蛋白偶联率605%,酶活性回收率55%;将其直接亲和层析牛肺提取液,分离纯化高比活抑肽酶。方法过程简单,样品比活力5700KIU/mg,质量稳定,成本较低;该吸附剂机械强度高,抗污染能力较强,非特异性吸附较小,可以反复使用,价格低廉,适合工业化生产。The trypsin was covalently linked with chemical modified granule chitosan and was used to isolate and purify aprotininum from the extract of cattle lungs by affinity chromatography.Then hingh-purity aprotininum was prepared after ultrafiltrating and freeze drying.The result showed:the specific activity of immobilized trypsin on chitosan was 25950KIU/mg,65.5% purity of aprotininum was high,and the activity reccvery of trypsin was 55%.The purity of aprotininum was high,and the activity teccvery of trypsin on immobilized trypsin had low non Specific adsoption and ideal anticontemination,and it could be used more than 72 times.It was accepted as a simple and stable method and suitable for purifying aprotininum with high activity in industrial manufactur-ing.

关 键 词：固定化酶 亲和层析 抑肽酶 壳聚糖 牛肺 

分 类 号：TQ464.8[化学工程—制药化工]