肝细胞生长因子对四氯化碳损伤肝细胞的保护作用及相关基因表达  

Protective effect of hepatocyte growth factor on hepatocyte poisoning by carbon tetrachloride and related gene expression in rats 

作　　者：邹原[1] 宫德正[1] 梅懋华[1] 张万琴[1] 

机构地区：[1]大连医科大学生理教研室,大连116027

出　　处：《生理学报》2000年第1期59-63,共5页Acta Physiologica Sinica

基　　金：SupportedbytheNationalNaturalScienceFoundationofChina! (No 39470 72 5)

摘　　要：利用无血清原代培养大鼠肝细胞 ,观察重组人肝细胞生长因子 (rhHGF)对CCl4染毒肝细胞的保护作用。结果表明 :( 1)rhHGF ( 5ng/ml)预处理后可显著提高CCl4( 15mmol/L)染毒肝细胞存活率 ,降低细胞内丙氨酸氨基转移酶 (ALT)、K+的漏出 ;( 2 )表皮生长因子 (EGF ,5 0ng/ml)和rhHGF ( 5ng/ml)合用预处理肝细胞 ,CCl4染毒后细胞内ALT、K+漏出较rhHGF和EGF单独保护组进一步降低 ;( 3 )大鼠肝部分切除和CCl4( 5 0 % ,2 5ml/kgbw)染毒后 ,再生肝内HGF基因及其受体基因 /c met的表达分别较假手术和盐水对照组显著升高。结果提示 ,rhHGF对CCl4染毒大鼠肝细胞具有保护作用 ;EGF和rhHGF有协同保护作用 ;Hepatocyte growth factor (HGF), as a pleiotropic factor, plays important roles in organ re^ge^ne^ration, organogenesis and morphogenesis, but few reports on protective effect of HGF on injured liver are available. The aim of this work was to investigate protective effects of recombinant human HGF (rhHGF) on intoxicated hepatocytes induced by carbon tetrachloride (CCl 4). The results are as follows: (1) rhHGF (5 ng/ml) could significantly increase the viability of hepatocytes intoxicated by CCl 4 (15 mmol/L), decrease the leakage of intracellular alanine transaminase (ALT) and potassium ions into the culture medium; (2) the combination of rhHGF (5 ng/ml) and epidermal growth factor (EGF) (50 ng/ml) could increase the viability further, decrease the leakage of intracellular ALT and potassium ions of hepatocytes; (3) high expression of HGF and its receptor/c met gene in regenerating liver after partial hepatectomy and CCl 4 (50%, 2 5 ml/kg bw) poisoning were examined respectively by reverse transcription polymerase chain reaction in rats. The results showed that rhHGF could protect hepatocytes against CCl 4 through preventing intracellular ALT and potassium ion leakage, thus suggesting a synergistic effect of rhHGF and EGF on cytoprotection, and high expression of HGF and its receptor/c met mRNA in the liver treated with PH and CCl 4 poisoning. The present study suggests that the expression of HGF and its receptor gene plays an important role in liver regeneration and repair.

关 键 词：C-MET基因 四氯化碳 肝细胞原代培养 rhHGF EGF 

分 类 号：R329.3[医药卫生—人体解剖和组织胚胎学]