橡胶树HbEBP1蛋白亚细胞定位初步研究  

The Subcellular Localization of Rubber Tree HbEBP1 Protein

作　　者：祝建顺[1,2] 程汉[1] 肖成江[1,2] 安泽伟[1] 黄华孙[1] 

机构地区：[1]中国热带农业科学院橡胶研究所国家橡胶树育种中心,海南儋州571737 [2]海南大学农学院,海南海口570228

出　　处：《热带作物学报》2013年第5期815-820,共6页Chinese Journal of Tropical Crops

基　　金：国家天然橡胶产业体系(No.CARS-34);中央级公益性科研院所基本科研业务费专项基金项目(No.1630022011002))

摘　　要：利用融合PCR法构建HbEBP1基因与绿色荧光蛋白基因的融合表达载体,成功转化拟南芥,借助激光共聚焦显微镜检测HbEBP1-EGFP融合蛋白在转基因植株根细胞中的表达及分布。结果显示:在转基因植株根中观察到强烈荧光信号,并分布于细胞质与细胞核中。结果证实了HbEBP1蛋白同时定位于细胞质与细胞核中。To investigate the subcellular localization of HbEBP1 and find the clues for its function, a HbEBP1- EGFP fusion protein expression vector was constructed by fusion PCR method, and was transformed into Arabidopsis thaliana. The roots of transgentic plant were observed under the confocal microscope for detecting the EGFP distribution. The green fluorescence signal was observed to distribute both in the cytosol and nuclear in transgenic Arabidopsis root cells. The results showed HbEBP1 was localized to both the cytosol and nuclear.

关 键 词：橡胶树 融合PCR HbEBP1蛋白 亚细胞定位 

分 类 号：S794.1[农业科学—林木遗传育种]