双波长RP-HPLC测定解毒祛瘀滋阴方阿魏酸、异阿魏酸和芍药苷的含量  

Simultaneous Determination of Ferulic Acid,Isoferulic Acid and Paeoniflorin in Jiedu Quyu Ziyin Recipe by Dual Wavelength RP-HPLC

作　　者：吕惠卿[1] 张小平[2] 涂继方[1] 李祖光[2] 温成平[1] 

机构地区：[1]浙江中医药大学,浙江杭州310053 [2]浙江工业大学,浙江杭州310014

出　　处：《中华中医药学刊》2013年第6期1245-1247,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(81173249);中国博士后科学基金资助项目(2012M511389)

摘　　要：目的:建立双波长反相高效液相色谱(RP-HPLC)同时测定解毒祛瘀滋阴方阿魏酸、异阿魏酸和芍药苷的含量。方法:色谱柱为Dionex C18柱(4.6 mm×250 mm,5.0μm),流动相A乙腈,流动相B为0.1%甲酸溶液(体积比),梯度洗脱,流速为0.8 mL.min-1,检测波长:阿魏酸和异阿魏酸325 nm,芍药苷为275 nm,柱温25℃。结果:阿魏酸、异阿魏酸和芍药苷的质量浓度分别在0.15～15.0μg.mL-1(r=0.9999)、0.25～25.0μg.mL-1(r=0.9994)和1.50～60.0μg.mL-1(r=0.9999)内与峰面积呈良好的线性关系(n=5),平均回收率(n=9)分别为99.1%,98.3%,100.1%。结论:该法操作简单,灵敏度高,可用于解毒祛瘀滋阴方阿魏酸、异阿魏酸和芍药苷的同时测定。Objective：To develop a dual wavelength RP - HPLC method for the simultaneous determination of ferulic acid,isoferulie acid and paeoniflorin in Jiedu Quyu Ziyin recipe. Method： The reversed phase HPLC system consisted of a Dionex C18 column（4.6 mm × 250 mm,5.0 um） by gradient elution using a mixture of acetonitrile-0.1% HAC as the mobile phase at a flow rate of 0.8 mL . min - 1. The UV detection wavelength absorbanees of ferulic acid , isoferulic acid and paeoniflorin were monitored at 325nm ,325 nm and 275 nm ,respectively and the column temperature was 25 ℃. Results ：There was good linearitv and the response ranges of ferulic acid. isoferulic acid and oaeoniflorin were 0.15 -15.0 ug . mL - 1 （ r = 0.9999 ） ,0.25 - 25.0 ug . mL - 1 （ r = 0.9994 ） and 1.50 - 60.0 ug . mL - 1 （ r = 0. 9999 ）, respectively （ n = 5 ）. The recoveries （ n = 9 ） were 99.1% ,98.3 % and 100.1% , respectively. Conclusion： The method is simple, sensitive and rapid, and could be used for simultaneous determination of ferulic aeid,isoferulie acid and paeoniflorin in Jiedu Quyu Ziyin recipe.

关 键 词：解毒祛瘀滋阴方 阿魏酸 异阿魏酸 芍药苷 反相高效液相色谱 

分 类 号：R284.3[医药卫生—中药学]