5-FU结合大黄■虫汤诱导胃癌细胞凋亡的实验研究  

Apoptosis of Gastric Cancer Cells Induced by Combination of 5-Fu and Dahuang Zhechong Decoction

作　　者：郑爱红[1] 

机构地区：[1]浙江省人民医院肿瘤二科,浙江杭州310014

出　　处：《中华中医药学刊》2013年第6期1364-1366,I0006,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省中医药管理局资助项目(2009CA047)

摘　　要：目的:探讨5-FU结合大黄虫汤诱导胃癌细胞凋亡的作用。方法:将人胃癌BGC—823细胞裸鼠移植瘤动物模型,随机分为4组(每组12只):5-FU结合大黄虫汤组,大黄虫汤组,5-FU组,生理盐水组。连续用药3周,用药结束后脱颈处死裸鼠,统计各组抑瘤率;流式细胞仪检测移植瘤细胞凋亡率,RT-PCR检测凋亡关键因子Caspase-3表达。结果:5-FU结合大黄虫汤组抑瘤率高于大黄虫汤组、5-FU组(P<0.01);5-FU结合大黄虫汤组胃癌细胞凋亡率明显高于大黄虫汤组、5-FU组(P<0.01,P<0.05),5-FU结合大黄虫汤组Caspase-3水平最高,与大黄虫汤组、5-FU组比较有显著差异(P<0.01,P<0.05)。结论:5-FU结合大黄虫汤能提高胃癌细胞凋亡率,抑制胃癌的增长。Objective： To study the apoptosis of gastric cancer cells induced by combination of 5 - Fu and Dahuang Zhechong Decoction. Methods： Xenotransplanted human gastric carcinoma model was established by BGC 823 cell line, and was divided into 4 groups randomly （ 12 for each group） ： 5 - Fu plus Dahuang Zhechong Decoction group, Dahuang Zhechong Decoction group, 5 - Fu group, saline group, treated with 3 weeks continuously. Tumor growth inhibitory rate of each group was calculated by the end of experiments when the animals were sacrificed, flow cytometry was for analyzing the apoptosis rate of xenotrasplanted, RT - PCR assays were used to detect the levels of Caspase - 3. Results ： Tumor growth inhibitory rate of 5 - Fu plus Dahuang Zhechong Decoction group was significantly higher than that of Dahuang Zhechong Decoction group or 5 - Fu group（ P 〈 0.01 ）. The apoptosis rare of 5 - Fu plus Dahuang Zhechong Decoction group was apparent than that of Dahuang Zhechong Decoction group or 5 - Fu group（ P 〈 0. 01, P 〈 0.05 ）. There were significant differences between three groups in the levels of Caspase - 3, the levels of Caspase - 3 was highest of 5 - Fu plus Dahuang Zhechong Decoction group, compared with Dahuang Zhechong Decoction group and 5 - FU group （P 〈 0.01, P 〈 0. 05 ）. Conclusion： 5 - Fu plus Dahuang Zhechong Decoction can significantly improve the apoptosis rate and inhibit the growth of gastric cancer cells.

关 键 词：胃癌 5-FU 大黄■虫汤 细胞凋亡 

分 类 号：R735.2[医药卫生—肿瘤]