一株螯合球菌HB-4的分离及多相分类鉴定  

Identification of Polyphasic Taxonomy for a Chelatococcus sp. HB-4

作　　者：吴刚[1] 刘洋[2] 李青[1] 杜慧竟[2] 游靖[1] 李红[2] 柯丛玉 张欣[2] 余吉良[1] 赵婷[2] 

机构地区：[1]华北油田采油工程研究院,任丘062552 [2]中国食品发酵工业研究院中国工业微生物菌种保藏管理中心,北京100027

出　　处：《生物技术通报》2013年第5期165-170,共6页Biotechnology Bulletin

基　　金：中国石油天然气股份有限公司华北油田分公司重大科技项目(2012-HB-z231);中国食品发酵工业研究院科技发展基金(博士基金)项目(2012KJFZ-BS-01)

摘　　要：自华北油田地层水中分离得到的细菌HB-4,对其进行形态学、生理生化特征鉴定、化学成分分析以及遗传特征测定,并结合16S rRNA基因序列分子生物学鉴定及系统发育学分析。结果表明,该菌株归属于螯合球菌属(Chelatococcussp.),并且可鉴定为大邱螯合球菌(Chelatococcus daeguensis)。其最适生长温度为37℃,主要极性脂为DPG、PE、PG及PC,醌为Q-10,主要脂肪酸组成分别为C18:1ω7c(47.01%)和C19:0cycloω8c(21.83%),HB-4具有氧化酶、接触酶、酯酶(C4)、类脂酯酶(C8)、白氨酸芳胺酶、缬氨酸芳胺酶、胱氨酸芳胺酶、胰蛋白酶、胰凝乳蛋白酶、酸性磷酸酶及萘酚-AS-BI-磷酸水解酶等酶活性。A bacterial strain HB-4 was isolated from stratum water in Huabei Oilfield,and identificated by polyphasic taxonomy basing on morphologic and physiologic methods,chemical composition analysis,genetic characteristics test and 16S rRNA gene phylogenetic analysis.The result showed that the strain was a member of the genus Chelatococcus,and was belong to the known species of Chelatococcus daeguensis.37℃ was its optimum growth temperature.The major polar lipids presented in strain HB-4 were DPG,PE,PG and PC.Major fatty acids were C18 ：1 ω7c（47.01%）and C19 ：0 cyclo ω8c（21.83%）,and ubiquinone Q-10 was the predominant quinone.HB-4 had enzyme activity of oxidase,catalase,esterase（C4）,Esterase lipase（C8）,leucine arylamidase,valine arylamidase,cystine arylamidase,trypsin,chymotrypsin,acid phosphatase and naphthol-AS-BI-phosphate hydrolase.

关 键 词：华北油田 地层水 大邱螯合球菌 HB-4 多相分类鉴定 

分 类 号：TE357.9[石油与天然气工程—油气田开发工程]