高压脉冲电场法快速提取黄花乌头中关附甲素的工艺研究  

Extracting technology of guanfubase A from Aconitum coreanum by high-voltage pulsed electric field method

作　　者：白永智[1] 李昌禹[1] 赵景辉[1] 王英平[1] 

机构地区：[1]中国农业科学院特产研究所,吉林吉林130000

出　　处：《中草药》2013年第10期1267-1270,共4页Chinese Traditional and Herbal Drugs

基　　金：吉林省自然科学基金资助项目(201115131);吉林市科技局项目(201222306)

摘　　要：目的研究高压脉冲电场法对黄花乌头中关附甲素的最佳提取工艺,为工业化生产提供可靠的实验数据和理论依据。方法采用正交试验设计优化高压脉冲电场法快速提取方法,HPLC法测定关附甲素的量。结果提取黄花乌头关附甲素的最佳提取工艺条件为以90%乙醇为提取溶剂,电场强度20 kV/cm,脉冲数为10,料液比1∶14,影响因素由大到小依次为电场强度、料液比、脉冲数;此法提取黄花乌头中的关附甲素提取率达到0.391%,浸膏得率为39.92%。结论本提取工艺可行、经济、省时、回收率高。Objective To optimize the extracting technology for guanfubase A from Aconitum coreanum by high-voltage pulsed electric field method and to provide the reliable experimental data and theoretical basis for the industrial production.Methods The content of guanfubase A was detected by HPLC in orthogonal test.Results The optimal formulation was as follows： high voltage of 20 kV/cm,impulsion frequency of 10,and solid-to-solvent ratio of 1∶14,with 90% ethanol as extracting solvent,and the influence factor in size was voltage,impulsion frequency,and solid-to-solvent ratio.The guanfubase A content and extract yield were up to 0.391% and 39.92% in A.coreanum,respectively.Conclusion The extracting technology is feasible and economic,which has the advantage of time-saving and high recovery rate.

关 键 词：黄花乌头 关附甲素 正交试验 高压脉冲电场 浸膏得率 

分 类 号：R284.2[医药卫生—中药学]