辛热药和苦寒药对阳虚大鼠的不同药性生物学表达特征研究  

Different biological expression characteristics of pungent-hot and bitter-cold herbs on Yang-deficiency rats

作　　者：刘欣[1] 张冰[2] 刘小青[2] 崔一然[2] 张晓萍[2] 谢菲[2] 

机构地区：[1]兰州大学基础医学院中西医结合研究所,甘肃兰州730000 [2]北京中医药大学中药学院,北京100029

出　　处：《中草药》2013年第10期1295-1298,共4页Chinese Traditional and Herbal Drugs

基　　金：国家重点基础研究发展计划("973"计划)项目(2007CB512605);高等学校博士学科点专项科研基金博导类资助课题(20100013110011);高等学校博士学科点专项科研基金新教师类资助课题(20120211120046);甘肃省科技计划资助(1208RJYA056);兰州大学自然科学类"中央高校基本科研业务费专项资金"自由探索项目-面上项目(lzujbky-2012-151;lzujbky-2012-153)

摘　　要：目的通过观察附子、仙茅、肉桂、黄柏、栀子对阳虚模型大鼠下丘脑-垂体-靶腺轴各项功能指标的影响,揭示在阳虚状态下辛热药、苦寒药不同药性的生物学表征特点。方法辛热药附子、仙茅、肉桂和苦寒药黄柏、栀子水煎液每天ig给予阳虚模型大鼠1次,连续给药7 d,检测各组大鼠血清中促甲状腺激素(TSH)、三碘甲腺原氨酸(T3)、甲状腺素4(T4)、17-羟皮质类固醇(17-OHCS)、皮质醇(COR)、睾酮(T)、雌二醇(E2)等指标的变化。结果附子、仙茅、肉桂组均能显著升高阳虚大鼠T3、17-OHCS、COR、T、T/E2值,降低E2指标;附子、肉桂组显著升高阳虚大鼠T4水平,肉桂组显著降低阳虚大鼠TSH水平,黄柏、栀子组显著降低阳虚大鼠COR水平,黄柏组显著降低阳虚大鼠E2指标。结论辛热药附子、仙茅、肉桂和苦寒药黄柏、栀子在干预阳虚大鼠时表现出不同的药性,其中辛热药附子、仙茅、肉桂对阳虚状态的下丘脑-垂体-靶腺轴指标具有明显调节作用,而苦寒药黄柏、栀子无明显作用。Objective To initially explore the drug biological characteristics of pungent-hot and bitter-cold herbs on the various functions of hypothalamus-hypophysis-target gland axises in Yang-deficiency rats,according to the pungent-hot herbs,Aconiti Lateralis Radix Praeparata（ALRP）,Curculiginis Rhizoma（CR）,Cinnamomi Cortex（CC）,and bitter-cold Phellodendri Chinensis Cortex（PCC）,and Gardeniar Fructus（GF） and to investigate their biological expression characteristic on Yang-deficiency state.Methods The decoctions of ALPR,CR,CC,PCC,and GF were ig administered to Yang-deficiency rats once daily for 7 d.The indexes of thyroid-stimulating hormone（TSH）,triiodothyronine（T3）,tetraiodothyronine（T4）,17-hydroxycorticosteroids（17-OHCS）,cortisol（COR）,testosterone（T）,and estradiol（E2） in serum of animals in each group were detected.Results The T3,17-OHCS,COR,T,and T/E2 values of Yang-deficiency rats in ALRP,CR,and CC groups were obviously increased,while E2was decreased.ALRP and CC could significantly increase T4 of Yang-deficiency rats,TSH in Yang-deficient rats was decreased by CC,the COR of Yang-deficiency rats in CC and GF groups was decreased,and E2 in Yang-deficiency rats was decreased by CC.Conclusion The pungent-hot herbs of ALRP,CR,and CC and bitter-cold herbs of PCC,GF show the different drug property in intervention of Yang-deficiency rats.The pungent-hot herbs have an obvious regulation on hypothalamus-hypophysis-target gland axises,while the bitter-cold herbs have no significant role.

关 键 词：辛热药 苦寒药 阳虚 药性 下丘脑-垂体-靶腺轴 

分 类 号：R285.5[医药卫生—中药学]