旋覆花素对AD模型大鼠脑海马组织Casepase-3表达影响  

EFFECT OF INULICIN ON EXPRESSION OF CASEPASE-3 IN AD MODEL RATS HIPPOCAMPUS

作　　者：王英杰[1,2] 王文胜[2] 苏军红[1] 柴锡庆[2,3] 刘志军[1] 

机构地区：[1]河北省邯郸市第一医院神经内科,河北邯郸056002 [2]河北医科大学基础医学研究所省部共建教育部重点实验室,河北石家庄050017 [3]河北化工医药职业技术学院新药研发基地,河北石家庄050026

出　　处：《河北医科大学学报》2013年第5期501-503,共3页Journal of Hebei Medical University

摘　　要：目的观察旋覆花素对β-淀粉样蛋白25～35片段(β-amyloid protein 25～35,Aβ_(25～35))海马内注射所致阿尔茨海默病(Alzheimers disease,AD)模型大鼠海马Caspase-3表达的影响,探讨其对AD大鼠的治疗作用及机制。方法 30只SD大鼠随机分为对照组、模型组及给药组,Aβ_(25～35)海马内注射制备AD大鼠模型,给药组给予旋覆花素26mg·kg^(-1)·d^(-1)、模型组给予等量溶剂,分2次灌胃,共21d。免疫组织化学及Western blot测定大鼠海马Caspase-3表达。结果模型组海马Caspase-3表达升高,给药组Caspase-3表达下降,与模型组比较差异有统计学意义(P<0.05)。结论旋覆花素主要通过抑制Caspase-3的表达,从而抑制Aβ诱导的AD模型大鼠脑海马的神经细胞凋亡。Objective To explore the molecular mechanism of inulicin in rats with Ahheimers disease （AD）. Methods Sprague-Dawley （SD） rats weighing of （364.5 ± 15.0） g were microinjected with β-amyloid protein 25 -35 （Aβ25-35） into the hippocampus to induce AD model rats. In inulicin treatment group, the animals were pretreated with inulicin （contain 1-o-acetylbritannilactone 2.66g/L） dissolved in tween-80 3 days before operation, then inulicin was administered to rats at dose of ABL 26mg · kg-1 . d- 1 by gastric gavage orally for 21 days （n = 10 ）. The casepase-3 protein in hippocampus was detected by immunohistochemistry and western blot. Results Casepase-3 expression was more intensive in hippocampus cell of model rats than that of control rats （P 〈 O. 05 ）, but there was no signifcant difference between innulicin-treated and control rats （P 〉 0.05）. Conclusion Inulicin could inhibit the apoptosis by Aβ injection into hippocampus by down-regulating casepase-3 expression.

关 键 词：阿尔茨海默病 淀粉样Β-蛋白 旋覆花素 大鼠 

分 类 号：R742[医药卫生—神经病学与精神病学]