Prx Ⅴ和CAT在大鼠肾缺血再灌注损伤模型的表达变化  

EXPRESSION OF Prx Ⅴ,CAT mRNA IN RENAL ISCHEMIA-REPERFUSION INJURY OF RATS

作　　者：孟彦丽[1] 王切[2] 王素玲[3] 苏玉红[2] 王真[4] 董辉[1] 

机构地区：[1]华北石油管理局总医院急诊科,河北任丘062552 [2]河北医科大学基础医学院解剖学教研室,河北石家庄050017 [3]河北省血液中心检验科,河北石家庄050071 [4]华北石油管理局总医院神经内科,河北任丘062552

出　　处：《河北医科大学学报》2013年第5期508-511,共4页Journal of Hebei Medical University

摘　　要：目的观察过氧化物氧化还原酶(peroxiredoxinⅤ,PrxⅤ)和过氧化氢酶(catalases,CAT)在大鼠肾缺血再灌注损伤模型的表达变化,探讨其在抗氧化应激反应中的作用。方法通过无损伤动脉夹钳夹肾动脉法建立肾缺血再灌注损伤模型。再灌注24h后取血和肾脏。苦味酸法和酶偶联速率法分别检测血清肌酐(serumcreatinine,SCr)、血尿素氮(blood urea nitrogen,BUN)浓度;HE法观察大鼠肾脏形态学改变,反转录聚合酶链反应(reverse transcription polymerase chain reaction,RT-PCR)方法观察PrxⅤ和CAT在肾脏缺血再灌注损伤中mRNA水平的表达变化。结果与对照组相比,肾缺血再灌注损伤组大鼠血清中SCr和BUN的含量明显升高。HE结果提示肾脏缺血再灌注损伤组大鼠的肾组织明显受损。PrxⅤ和CAT mRNA水平也明显升高。结论 PrxⅤ和CAT在肾脏缺血再灌注损伤中均发挥了抗氧化应激作用。Objective To observe the expression of peroxiredoxin V （Prx V ） ,catalases （CAT）＇ in renal ischemia-reperfusion injury of rats and study their role in oxidative-stress response. Methods The renal artery of rats was clipped with non-damage vascular clamp to creat renal ischemia-reperfusion injury model. After 24 hours of reperfusion, the blood and kidney were taken. The serum creatinine （SCr） and blood urea nitrogen （BUN） were detected with picric acid method and enzyme coupling rate method, the morphology change was observed by HE staining. The PrxV and CAT mRNA expression were evaluated by reverse transcription polymerase chain reaction （RT-PCR）. Results Compared with the control group, SCr and BUN of renal ischemia-reperfusion injury group were significantly increased. The HE staining results of renal ischemia-reperfusion injury group showed that kidney was impaired. The Prx V and CAT mRNA expression were also enhanced. Conclusion Prx V and CAT may play an important role in inhibiting the oxidative-stress response in the process of renal ischemia-repcrfnsion injury.

关 键 词：再灌注损伤 过氧化氢酶 氧化性应激 

分 类 号：R364.1[医药卫生—病理学]