Differential expression of microRNA clusters in bladder transitional cell carcinoma  

膀胱移行细胞癌microRNAs差异表达及意义(英文)

作　　者：Feng Xu Zhifeng Wei Zhengyu Zhang Jingping Ge Peng Xie Hongqing Ma Jianping Gao Wen Cheng 

机构地区：[1]Department of Urology,Jinling Hospital [2]Nanjing General Hospital of Nanjing Military Command,PLA

出　　处：《The Chinese-German Journal of Clinical Oncology》2013年第6期285-289,共5页中德临床肿瘤学杂志（英文版）

基　　金：Supported by a grant of National Natural Science Foundation of China (No.30772278)

摘　　要：Objective： The aim of the study was to investigate the differential expression of microRNAs （miRNAs） in bladder transitional cell carcinoma （BTC）. Methods： Fresh tissues were obtained from patients with BTC （9 cases; 3 cases with grade Ⅰ, 3 cases with grade Ⅱ, 3 cases with grade III） and those with normal bladder mucosa （3 cases） and stored in liquid nitrogen. Total RNA was extracted using TRizol reagent and RNA was quantified and quality control was performed, miRNA probes were labeled with Hy3TM fluorescence, then hybridized with a miRCUR^M array labeling kit. miRNAarrays were scanned and analyzed and the scanned result was validated using reverse transcription-polymerase chain reaction （RT-PCR）. Results： In four groups of differentially expressed genes obtained from grade Ⅰ, grade Ⅱ, grade Ⅲ, and grade Ⅰ ＋ grade Ⅱ ＋ grade Ⅲ BTC tissues compared with normal bladder mucosa, hsa-miR-29b-1＊ was upregulated, and hsa-miR-923 and hsa-miR-300 were downregulated. The hsa-miR-29b-1＊, hsa-miR-300, and hsa-miR-923 findings were confirmed by real-time RT-PCR. Conclusion： Genes that were differentially expressed between BTC and normal bladder mucosa may be involved in the pathogenesis and development of BTC, and may be useful for further studies of BTC-related genes.

关 键 词：bladder transitional cell carcinoma （BTC） microRNAs （miRNAs） differential expression 

分 类 号：R737.14[医药卫生—肿瘤]