MK-801诱导出生早期大鼠精神分裂症模型的行为学研究  

Study on the behavior of rat model of schizophrenia induced by MK-801 in early postnatal period

作　　者：蔡菡[1] 李泽爱[2] 刘寰忠[2] 任振华[3] 

机构地区：[1]安徽医科大学医学心理学系,合肥230032 [2]安徽省精神卫生防治中心,合肥230022 [3]安徽医科大学解剖学教研室,合肥230032

出　　处：《安徽医科大学学报》2013年第7期768-771,共4页Acta Universitatis Medicinalis Anhui

基　　金：合肥科技局基金(编号:[合科]2011-25-62)

摘　　要：目的建立地卓西平马来酸盐(MK-801)慢性处理的出生早期大鼠精神分裂症(SZ)模型,探讨其神经行为学变化。方法 11只出生后6 d的SD仔鼠,腹腔注射0.3 mg/kg的MK-801(MK-801组),一天2次,连续注射2周;11只同龄的SD仔鼠腹腔注射等体积的生理盐水(NS组)。给药前称重1次,给药完毕后每隔2 d称重1次,直至实验结束。给药后1周,检测旷场和水迷宫等行为学的变化。结果与NS组比较,MK-801组大鼠体重显著降低(P<0.01),并至少持续至注射结束后4周。行为学检测结果显示,与NS组比较,MK-801组大鼠自发活动显著减少(P<0.01),其学习能力明显降低,学习速度减慢,空间记忆能力下降(P<0.05)。结论 MK-801诱导的出生早期SZ模型大鼠自发活动减少,能部分模拟SZ的阴性症状;其学习及记忆功能损害可以部分模拟SZ的认知功能损害症状。Objective To study the learning and memory characteristic of rat models of schizophrenia induced by chronic MK-801 treatment in the early postnatal period. Methods 22 SD rats 6 d after birth were divided into MK- 801 group and normal saline group of 11 each, and the rats in the two groups accepted intraperitoneal administra- tion of MK-801 （0. 3 mg/kg） or saline （0. 3 ml/kg） twice a day for 2 weeks, respectively. Body weights of rats in two groups were measured before and every three days after injection, and continued up to the end of the experi- ment. After 1 week of injection endpoint, open field and water maze were detected in the rats of two groups. Re- suits Compared with the normal group, body weights of rats in MK-801 group were gradually decreased during the injection process （P 〈 0. 01 ） and at least continued for 4 weeks after the end of injection. The results of behavior showed that, compared with the normal saline group, the spontaneous activity in rats of MK-801 group significantly reduced （ P 〈 0. 01 ）, and the ability of learn and memory significantly were declined in rats of MK-801 group （ P 〈 0.05 ）. Conclusion The decrease in the spontaneous activity of rat model of schizophrenia induced by MK-801 in the early postnatal period could partially imitate the negative symptoms of schizophrenia, and the dysfunction in learning and memory is similar to the cognitive impairment of schizophrenia.

关 键 词：MK-801 精神分裂症 动物模型 大鼠 行为学 

分 类 号：R749.3[医药卫生—神经病学与精神病学]