用高效液相色谱法测定血浆中的阿苯达唑及其主要代谢物  

Determination of Albendazole and Its Metabolites in Plasma by High Performance Liquid Chromatography

作　　者：王鸣杰[1] 肖树华[1] 黄立信[1] 

机构地区：[1]中国预防医学科学院寄生虫病研究所

出　　处：《地方病通报》1991年第3期7-11,共5页Endemic Diseases Bulletin

摘　　要：用高效液相色谱法测定血浆中的阿苯达唑(Alb)及其代谢物阿苯达唑亚砜(AlbSO)和阿苯达唑砜(AlbSP)。检测血浆中的Alb和AlbSO的限度为5ng·ml^(-1),AlbSP的为10ng·ml^(-1)。应用本法检测Alb、AlbSO和AlbSP的回收率均在90％以上。A high performance liquid chromatography method was used for quantitative determination of albendazole (Alb) and its major metabolites, albendazole sulfoxide (AlbSO) and albendazole sulphone (AlbSP) in plasma of rabbits and mice. A ODS (5μm) column ditted with a variable wavelength UV detector at 292 nm was used for assay, and mebendazole was used as internal standard. The mobile phase used was 48% methanol / H2O (v / v, containing HClO4 0.6 ml·L-1) with a flow rate of 1 ml. min-1. The detectable limitation of Alb or AlbSO in plasma was 5 ng·ml-1, while that of AlbSP was 10 ng·ml-1. The recoveries of Alb, AlbSO and AlbSP by the method were 93.5%, 96.6% and 99.2%, respectively. The results showed that the method is simple, precise and sensitive and is suitable for pharmacokinetic study and microdetermination of the relevant substances present in the tissues.

关 键 词：高效液相色谱法 阿苯达唑 

分 类 号：R914.1[医药卫生—药物化学]