大肠癌动物模型的建立与中药干预作用  

The Establishment of Animal Model for Colorectal Cancer and the Intervention Effect of Traditional Chinese Medicine

作　　者：吴德坤[1] 莫启章[1] 黄瑞诚[2] 

机构地区：[1]广西中医药大学附属瑞康医院,广西南宁530011 [2]广西中医药大学,广西南宁530001

出　　处：《中国医药指南》2013年第11期448-449,共2页Guide of China Medicine

摘　　要：目的探究大肠癌动物模型的建立及中药(加味痛泻要方)的干预作用。方法将100只小鼠作为研究对象,分为四组(加味痛泻要方剂量高中低三组+对照组),初期给药(DMH)诱发小鼠大肠癌,后采用免疫组化法对四组小鼠突变型P53蛋白表达进行观察,同时给予中药进行后期干预并做好观察记录。结果加味痛泻要方组发癌率明显低于对照组,并且中药干预能明显降低突变型P53蛋白表达。结论中药干预作用明显,对防治大肠癌疗效突出。Objective To explore the establishment of animal model for colorectal cancer and the intervention effect of traditional chinese medicine （flavored pain diarrhea Yaofang）. Methods 100 mice as the research object, divided into four groups （flavored pain diarrhea Yaofang dose professinally three groups and control group）, initial dose （DMH） bath after colorectal cancer in mice induced by immunohistochemical method, four mice mutant P53 protein expression observation, at the same time give chinese medicine for later intervention and make observation record. ResuRs Flavored pain diarrhea Yaofang group hair cancer rate was significantly lower than the control group, and traditional chinese medicine intervention can significantly reduce the mutant P53 protein expression. Conclusion Chinese medicine intervention has an obvious effect in the prevention of coloreetal cancer, curative effect prominent.

关 键 词：大肠癌 小鼠 中药干预 加味痛泻要方 

分 类 号：R735.34[医药卫生—肿瘤]