PPAR-γ通路在尼美舒利抑制胃癌小鼠癌细胞生长中的作用  

Role of PPAR-γ Pathway in Tumor Growth Inhibition Induced by Nimesulide in Mice with Gastric Cancer

作　　者：唐涛[1] 费素娟[2] 刘军权[3] 陈复兴[3] 李伟平[1] 

机构地区：[1]湖州市中心医院,浙江湖州313000 [2]徐州医学院附属医院,江苏徐州221000 [3]中国人民解放军第九七医院,江苏徐州221000

出　　处：《肿瘤学杂志》2013年第5期327-331,共5页Journal of Chinese Oncology

基　　金：江苏省徐州市科技局社会发展项目(X20052321)

摘　　要：[目的]研究尼美舒利(NIM)是否通过过氧化物酶增殖物激活受体γ(PPAR-γ)途径影响胃癌细胞体内生长。[方法]通过培养人胃癌SGC-7901细胞,建立裸鼠胃癌移植瘤模型。设立GW9662+NIM组、生理盐水组、GW9662组、NIM组共4组。3周后测量各组裸鼠移植瘤体积,并采用免疫组化法检测裸鼠移植瘤组织PPAR-γ表达。[结果]NIM组裸鼠移植瘤体积较生理盐水组明显小,而GW9662+NIM组较NIM组移植瘤体积大,各组之间差异有显著性(P<0.05)。GW9662+NIM组与NIM组相比,移植瘤组织的PPAR-γ蛋白表达降低。[结论]尼美舒利在体内能抑制胃癌细胞的生长,PPAR-γ通路可能在此过程中发挥重要作用。[Purpose] To study the role of PPAR-γ pathway in tumor growth inhibition induced by nimesulide（NIM） in stomach cancer cells in vivo.[Methods] Nude BABL/c mice were implanted with SGC-7901 as human gastric cancer xenograft model,which were randomly divided into 4 groups：control group,GW9662 group,NIM group and GW9662＋NIM group.The volume of transplantation tumors was measured and the expression of PPAR-γ protein was detected by immunohistochemistry after three weeks.[Results] The volume of transplantation tumors in nude mice in NIM group was smaller than that in the control group,but the volume of transplanted tumor in GW9662＋NIM group was bigger than that in the NIM group.There was signifi-cantly different among the 4 groups（P0.05）.Compared with NIM group,the expression of PPAR-γ protein in transplanted tumor decreased obviously in GW9662＋NIM group.[Conclusion] Nimesulide is capable of inhibiting tumor growth in nude mice.PPAR-γ pathway may play an important role in this process.

关 键 词：胃肿瘤 移植瘤 PPAR-Γ 尼美舒利 肿瘤生长 

分 类 号：R73-754[医药卫生—肿瘤]