胃癌组织中p16基因甲基化状态及临床意义  

Methylation Status of p16 Gene in Gastric Cancer Tissue and Its Clinical Significance

作　　者：吕萍[1,2] 凌志强[1,3] 余江流[1] 韩静[1] 王实[1] 王新宝[1] 余齐鸣[1] 方铣华[1] 

机构地区：[1]浙江省肿瘤医院,浙江省肿瘤研究所,浙江杭州310022 [2]舟山市人民医院,浙江舟山202451 [3]浙江省胸部肿瘤(肺,食管)诊治技术研究重点实验室,浙江杭州310022

出　　处：《肿瘤学杂志》2013年第5期332-335,共4页Journal of Chinese Oncology

基　　金：浙江省科技计划资助项目(2009C33143);浙江省医药卫生平台骨干人才计划项目(2011RCA009)

摘　　要：[目的]探讨胃癌患者癌组织、癌旁组织以及对照正常胃黏膜组织中p16基因的甲基化状态,及其与临床病理参数及预后的关系。[方法]应用甲基化特异性实时荧光聚合酶链反应技术检测92例胃癌患者癌组织及配对癌旁组织中p16基因启动子区5′-CpG岛甲基化状态,分析p16基因异常甲基化与患者临床病理特征以及预后之间的关系。[结果]胃腺癌患者癌组织中检测到p16基因甲基化79例(85.9%),癌旁组织11例(12.0%),胃炎患者组织10例(20.8%),健康人未检测到甲基化。p16基因甲基化与肿瘤大小、分化程度、是否淋巴管侵犯、T分期、有无淋巴结转移、有无远处转移、临床分期有关(P<0.05)。p16基因甲基化影响患者预后,但不是胃癌患者无病生存的独立预后因子。[结论]胃腺癌患者p16基因甲基化与肿瘤的生物学行为有关,提示肿瘤进展,恶性程度高,预后欠佳。[Purpose] To investigate the aberrant methylation status of p16 gene in gastric adenocarcinoma tissues,normal gastric mucosa and cancer adjacent normal tissues,and the relationship with clinicopathological characteristics and prognosis.[Methods] The methylation status of p16 gene 5＇-CpG island in gastric adenocarcinoma tissue and paired cancer adjacent tissue in 92 gastric cancer patients were examined by real-time methylation-specific polymerase chain reaction（qMSP）.The relationships of p16 gene methylation with clinicopathologic features or prognosis were analyzed.[Results] Seventy-nine cases（85.9%） of cancer tissue had p16 gene methylation;11 cases（12.0%）,cancer adjacent tissue;10 cases（20.8%）,gastritis patients;and 0 case,healthy control.p16 gene methylation correlated with tumor size,degree of differentiation,lymphatic vessel invasion,T stage,lymphatic metastasis,clinical stage and distant metastasis（P0.05）.[Conclusion] Ectopic methylation of p16 gene in gastric adenocarcinoma correlates with tumor biological behavior.p16 gene methylation affects the prognosis of patients with gastric adenocarcinoma,but it is not an independent predictor of disease-free survival.

关 键 词：胃肿瘤 P16基因 甲基化 

分 类 号：R735.2[医药卫生—肿瘤]