神经胶质瘤患者外周血淋巴细胞亚群检测的临床意义  

Clinical significance of peripheral blood lymphocyte subsets detection in patients with glioma

作　　者：马学华[1] 张萱[1] 滕雷[2] 关秀茹[1] 崔兰英[1] 韩伟[3] 

机构地区：[1]哈尔滨医科大学附属第一医院检验科,黑龙江哈尔滨150001 [2]哈尔滨医科大学附属第一医院神经外科,黑龙江哈尔滨150001 [3]哈尔滨医科大学附属第一医院病理科,黑龙江哈尔滨150001

出　　处：《国际检验医学杂志》2013年第9期1080-1082,共3页International Journal of Laboratory Medicine

基　　金：黑龙江省教育厅基金资助项目(11521105)

摘　　要：目的检测神经胶质瘤患者外周血淋巴细胞亚群及CD4+CD25+调节性T淋巴细胞(Treg),探讨其与胶质瘤发病的关系。方法将86例神经胶质瘤患者作为胶质瘤组,52例健康体检者作为对照组。采用流式细胞术检测神经胶质瘤患者及健康人外周血中CD3+CD4+、CD3+CD8+、CD4+/CD8+、CD3+HLA-DR+、CD3+T淋巴细胞,CD3-CD19+B淋巴细胞,CD3-CD16+56+自然杀伤细胞(NK)及CD4+CD25+Treg水平。结果与对照组比较,胶质瘤组患者外周血中CD3+CD4+、CD3+HLA-DR+及CD4+/CD8+T淋巴细胞百分比明显降低(P<0.05)。与低级别组比较,高级别组胶质瘤患者外周血中上述T淋巴细胞百分比明显降低(P<0.05),CD3+CD8+T淋巴细胞百分比升高(P<0.05)。与对照组相比,胶质瘤患者外周血CD3-CD19+B淋巴细胞、CD3-CD16+56+NK的百分比明显降低(P<0.05),CD4+CD25+Treg百分比明显升高(P<0.05)。与低级别组比较,高级别胶质瘤患者外周血CD3-CD16+56+NK百分比明显降低(P<0.05),CD4+CD25+Treg百分比明显升高(P<0.05)。结论淋巴细胞亚群的改变和CD4+CD25+Treg水平的升高与恶性肿瘤患者免疫功能低下及肿瘤的发生、发展密切相关。Objective To detect lymphocyte subsets and CD4＋CD25＋ regulatory T lymphocytes（Treg） in peripheral blood from patients with glioma and investigate their relationship with glioma onset.Methods Eighty-six patients with glioma served as glioma group and fifty-two healthy people as control group.Flow cytometry was used to detect the levels of CD3＋CD4＋,CD3＋CD8＋,CD4＋/CD8＋,CD3＋HLA-DR＋,CD3＋ T-lymphocytes and CD3-CD16＋56＋ natural killer cells（NK）,as well as CD4＋CD25＋Treg in peripheral blood in patients with glioma and healthy people.Results Compared with control group,percentages of CD3＋CD4＋,CD3＋HLA-DR＋ and CD4＋/CD8＋ T-lymphocytes in peripheral blood of patients in glioma group were decreased significantly（P＆lt;0.05）.Percentages of T-lymphocytes above in high-grade glioma group were reduced markedly（P＆lt;0.05）,while that of CD3＋CD8＋ T-lymphocytes was increased（P＆lt;0.05） when compared with low-grade glioma group.Compared with control group,percentages of CD3-CD19＋ B-lymphocytes and CD3-CD16＋56＋NK in peripheral blood of patients in glioma group were decreased significantly（P＆lt;0.05）,while that of CD4＋CD25＋Treg was increased（P＆lt;0.05）.Percentage of CD3-CD16＋56＋NK in high-grade glioma group was reduced obviously（P＆lt;0.05）,while that of CD4＋CD25＋Treg was increased significantly（P＆lt;0.05） when compared with low-grade glioma group.Conclusion Alteration of lymphocyte subsets and increasing level of CD4＋CD25＋Treg are closely related to immune dysfunction of patients with malignancy and tumor occurrence and development.

关 键 词：神经胶质瘤 流式细胞术 淋巴细胞亚群 T淋巴细胞 调节 

分 类 号：R739.4[医药卫生—肿瘤]