饱和脂肪饮食对豚鼠学习记忆、脑β-淀粉样蛋白及氧化应激的影响  

Effect of saturated fat diet on learning and memory,brain beta-amyloid and oxidative stress of guinea pigs

作　　者：翟伟科[1,2] 王小引[1] 樊振琦[2] 王朋[2] 张天[2] 闫金库[2] 马驰[2] 冯汉[2] 王冰[2] 杨彩玲[3] 石如玲[1] 

机构地区：[1]新乡医学院生物化学与分子生物学教研室,河南新乡453003 [2]新乡医学院基础医学院,2010级本科生河南新乡453003 [3]新乡医学院第一附属医院口腔颌面外科,河南新乡453100

出　　处：《新乡医学院学报》2013年第6期429-431,共3页Journal of Xinxiang Medical University

基　　金：新乡医学院高学历人才科研启动项目(编号:08BSKYQD-006);新乡医学院大学生科研创新计划项目(编号:DXSKYKT2011-010)

摘　　要：目的观察饱和脂肪饮食对豚鼠学习记忆、脑β-淀粉样蛋白(Aβ)、总超氧化物歧化酶(T-SOD)及丙二醛(MDA)的影响。方法 24只雄性豚鼠随机分为对照组和饱和脂肪组,每组12只。对照组给予正常饲料,饱和脂肪组饲料中添加质量分数15%牛油,喂养16周。用Morris水迷宫测定豚鼠空间学习记忆能力,用酶联免疫吸附分析法测定海马Aβ水平,用黄嘌呤氧化酶法测定脑组织T-SOD,用硫代巴比妥酸法测定脑组织MDA水平。结果与对照组比较,饱和脂肪组豚鼠的平均逃避潜伏期显著延长(P<0.05),穿越原平台位置的次数显著减少(P<0.05),海马Aβ水平显著升高(P<0.05),脑T-SOD活性显著降低(P<0.05)。结论饱和脂肪饮食引起学习记忆受损及Aβ增多,其机制可能与脑抗氧化能力降低有关。Objective To investigate the effect of saturated fat diet on learning and memory, brain beta-amyloid （ Aβ）, total superoxide dismutase （T-SOD） and malondialdehyde （MDA） levels in guinea pigs. Methods Twenty-four males guinea pigs were randomly divided into control group and saturated fat group, 12 guinea pigs in each group. The control group was given a normal diet ,and saturated fat group was given saturated fat diet containing 15% butter for 16 weeks. The spatial learning and memory abilities were examined by Morris water maze test. Aβ content in hippocampus was measured by enzyme linked immunosorbent assay. T-SOD activity was measured by using xanthine oxidase method. MDA content was determined by thiobarbiturate method. Results Compared with control group, the mean escape latency was prolonged and the frequency of crossing the original platform was reduced in saturated fat group （ P 〈 0.05 ） ; hippocampal Aβ content was increased significantly in saturated fat group（ P 〈 0.05 ） ; the brain T-SOD activity was decreased in saturated fat group （ P 〈 0.05 ）. Conclusion Saturated fat induces learning and memory impairment and Aβ increase,and its mechanism may be related to the decrease of brain antioxidant capacity.

关 键 词：饱和脂肪 学习记忆 Β-淀粉样蛋白 超氧化物歧化酶 阿尔兹海默病 

分 类 号：R741[医药卫生—神经病学与精神病学]