重组鼠源GM-CSF基因单纯疱疹病毒对小鼠胰腺癌PANC-2细胞的杀伤作用  

Experimental study on inhibits the proliferation of mouse pancreatic carcinoma cells panc-2 by oncolytic HSV^(GM-CSF) in vitro

作　　者：刘昊[1] 田艳涛[1] 解亦斌[1] 李秉珅 陈应泰[1] 崔亮[1] 

机构地区：[1]中国医学科学院北京协和医学院肿瘤医院腹部外科,北京100021 [2]中国人类基因组北方研究中心,北京100176

出　　处：《中国医刊》2013年第6期24-26,共3页Chinese Journal of Medicine

基　　金：北京希望马拉松专项基金(LC2012A09)

摘　　要：目的检测重组鼠源GM-CSF基因的单纯疱疹病毒(HSV^(GM-CSF))对于鼠胰腺癌PANC-2细胞的杀伤能力。方法体外培养鼠源胰腺癌PANC-2细胞,采用MTT检测不同浓度整合鼠源HSV^(GM-CSF)对PANC-2细胞的抑制情况。结果 MTT观察到不同浓度整合鼠源HSV^(GM-CSF)能够明显抑制PANC-2细胞生长,并且存在明显的量效反应关系。结论重组鼠源HSV^(GM-CSF)对PANC-2细胞生长有抑制作用。Objective To investigate the inhibitory effects of HSV^GM-CSF on the proliferation of mouse pancreatic canc-er cells and explore the possible mechanism. Method MTT assay was used to determine the cell growth of mouse pancreatic cancer cell line PANC-2 in vitro treated with the drug. Result HSV^GM-CSF treatment significantly sup-pressed the proliferation of panc-2 cells in a concentration-dependent manner compared with the control group. Con-clusion HSV^GM-CSF can suppress the proliferation of pancreatic carcinoma cell in vitro.

关 键 词：胰腺癌 基因治疗 单核细胞粒细胞集落刺激因子 单纯疱疹病毒 

分 类 号：R735.9[医药卫生—肿瘤]