食物中毒鸭沙门菌的生物学特性及分子分型研究  

Study on biological traits and molecular types of salmonella anatum from food poisoning

作　　者：汪永禄[1] 沈荣柴 王多春[3] 陶勇[1] 王利[1] 王艳[1] 刘力彰[3] 娄静[3] 闫梅英[3] 阚飙[3] 

机构地区：[1]马鞍山市疾病预防控制中心,安徽243000 [2]安徽当涂县疾病预防控制中心 [3]中国疾病预防控制中心传染病预防控制所传染病预防控制国家重点实验室

出　　处：《医学动物防制》2013年第5期486-489,492,共5页Journal of Medical Pest Control

摘　　要：目的对一起食物中毒分离的鸭沙门菌进行生物学特性和分子分型研究。方法菌株分离、生化鉴定和血清型确定参照GB/T4789.4-2010方法进行,用VITEK-32全自动微生物鉴定系统检测菌株药物敏感性,鲎试验检测内毒素、PCR检测沙门菌侵袭相关基因invA和invE,用脉冲场凝胶电泳(pulsed field gel electrophoresis,PFGE)方法进行分子分型。结果 11份样品检出8株肠炎沙门菌;其中5株菌内毒素为阳性;所有菌株均携带侵袭相关基因invA和invE;该次食物中毒样品中分离得到的8株鸭沙门菌PFGE图谱完全相同,但有别于其它地区分离的鸭沙门菌。结论 PFGE可有效用于沙门菌食物中毒细菌同源性的分析。本次食物中毒有相同的鸭沙门菌克隆系来源。Objective To study the biological traits and molecular types of salmonella anatin from a food poisoning.Method Do strains isolation,biochemical identification and serotypes according to GB/ T4789.4-2010.Detect the drug sensitivity by VITEK-32 automated microbial identification system,detect endotoxin by limulus test,detect salmonella invasion-related gene invA and invE by PCR,and type the moleculars pulsed field gel electrophoresis（PFGE） method.Results Eight salmonella were isolated from the 11 samples,among which,5 strains of bacteria endotoxin iwere positive,and all of them were salmonella anatis.All the strains carried invasion-related gene（invA and invE）.The PFGE maps of the 8 strains were the same,but different from that of other regions.Conclusions PFGE could be effectively used for homology analysis of salmonella food poisoning.This food poisoning had the same salmonella anatis clone source system.

关 键 词：食物中毒 鸭沙门菌 脉冲场凝胶电泳 分子分型 

分 类 号：R378.22[医药卫生—病原生物学] R155.3[医药卫生—基础医学]