氢气对中波紫外线致皮肤成纤维细胞氧化损伤的影响  

Effects of hydrogen on ultraviolet B-induced oxidative damage to skin fibroblasts

作　　者：邢卫斌 付国俊 叶文静 秦兰英 陈红光[2] 孟啸寅[2] 孟晨阳[3] 

机构地区：[1]沧州市人民医院皮肤科,河北061000 [2]天津医科大学 [3]沧州医学高等专科学校

出　　处：《中华皮肤科杂志》2013年第6期424-426,共3页Chinese Journal of Dermatology

摘　　要：目的探讨氢气对中波紫外线（UVB）致人成纤维细胞氧化损伤的影响。方法原代培养人成纤维细胞，分组如下：正常对照组，未经任何处理；氢气对照组，富氢培养液处理；UVB照射组；富氢培养液后处理组；富氢培养液预处理组。通过噻唑蓝（MYr）法观察细胞增殖情况，通过化学发光和酶联免疫吸附试验（ELISA）观察超氧化物歧化酶（SOD）、过氧化氢酶（CAT）的活性，丙二醛（MDA）和8异前列腺素2a（8-isoPGE20t）的水平，Western印迹观察血红素加氧酶-1（HO-1）蛋白的表达情况，采用单因素方差分析进行统计学处理。结果给予不同剂量的UVB照射，细胞增殖活性（A490）呈剂量依赖性降低。氢气后处理和预处理组细胞增殖活性明显高于相应UVB组（均P〈0．05）。氢气后、预处理组SOD和CAT活性以及HO-1蛋白表达水平明显高于UVB组（均P〈0．05），但MDA和8-iso—PGE2ct明显低于UVB组（均P〈0．05）。结论氢气可以减轻UVB诱发的成纤维细胞氧化损伤。Objective To observe the effect of hydrogen on ultraviolet B （UVB）-induced oxidative damage to skin fibroblasts. Methods Primary human skin fibroblasts from foreskin tissues were divided into five groups： normal control group receiving no treatment, hydrogen control group treated with hydrogen-rich saline, UVB group receiving irradiation only, post-treatment group irradiated with UVB followed by hydrogen-rich saline treatment, and pre-treatment group treated with hydrogen-rich saline followed by UVB irradiation. The dose of UVB was 30, 60 and 90 m J/era2 in the cell proliferation assay and 90 mJ/cm2 in the other experiments. Methyl thiazolyl tetra- zolium （MTF） assay was performed to evaluate the proliferative activity of fibroblasts, a chemiluminescence method to estimate the activity of superoxide dismutase （SOD） and catalase as well as to determine the level of malondialdehyde in the culture supernatant of fibroblasts, enzyme linked immunosorbent assay （ELISA） to determine the supernatant level of 8-isoprostane-prostaglandin F2a （8-iso-PGF2a）, Western blot to detect the expression of heine oxygenase-1 （HO-1） in fibroblasts. One-factor analysis of variance was conducted to assess differences in these parameters among these groups. Results UVB irradiation decreased the proliferative activity （absorbence value at 490 nm） of fibroblasts in a dose-dependent manner. Both the pre-treatment group and post-treatment group showed a statistical increase in proliferative activity of cells compared with the corresponding UVB control groups （all P 〈 0.05）. The activity of SOD and catalase as well as the protein expression of HO-1 were signifi- cantly higher （all P 〈 0.05）, whereas the supernatant levels of malondialdehyde and 8-iso-PGF2a were statistically lower （both P 〈 0.05） in the pre-treatment group and post-treatment group than in the UVB control group. Conclusion Hydrogen may mitigate UVB-induced oxidative damage to skin fibroblasts.

关 键 词：人成纤维细胞 细胞氧化损伤 中波紫外线 氢气 WESTERN印迹 细胞增殖活性 蛋白表达水平 UVB照射 

分 类 号：R[医药卫生]