幼年复发性呼吸道乳头状瘤儿童血清中IL-4和IFN-γ及IL-32的表达水平  

Levels of serum IL-4,IFN-γ,IL-32 in children with juvenile-onset recurrent respiratory papillomatosis

作　　者：南奔宇[1] 陈波蓓[1] 张初琴[1] 江明华[2] 章誉耀[1] 项海杰[1] 余啸[1] 

机构地区：[1]温州医学院附属二院耳鼻咽喉科,浙江温州325027 [2]温州医学院附属二院实验室

出　　处：《临床耳鼻咽喉头颈外科杂志》2013年第12期651-653,共3页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：温州市科技局对外合作项目(No:H20070039)

摘　　要：目的:研究幼年复发性呼吸道乳头状瘤(JORRP)患儿血清中IL-4、IFN-γ、IL-32的表达水平与JORRP发病机制的关系。方法:通过ELISA法检测15例JORRP患儿血清的IL-4、IFN-γ、IL-32的表达水平并进行统计学分析。结果:JORRP组血清IL-4浓度(524.65±147.77)pg/ml显著高于正常对照组(213.27±87.48)pg/ml(P<0.01);JORRP组血清IFN-γ浓度(2.87±0.84)pg/ml低于正常对照组(10.63±5.09)pg/ml(P<0.01);JORRP组血清IL-32浓度(2.47±1.60)pg/ml低于正常对照组(9.08±2.66)pg/ml(P<0.01)。结论:①Th2类细胞因子IL-4在JORRP患儿血清中浓度升高,而Th1类细胞因子IFN-γ浓度下降,JORRP患儿外周循环中Th1/Th2细胞免疫失衡;②JORRP患儿Th1/Th2细胞免疫失衡可能引起促炎因子IL-32浓度的降低,不能产生足够的炎症刺激引起免疫反应清除人乳头状瘤病毒。Objective：To explore the relationship between serum IL-4,IFN-γ,IL-10 levels and the aetiology of juvenile-onset recurrent respiratory papillomatosis.Method：Serum IL-4,IFN-γ,IL-32 levels of 15 JORRP children were detected by use of enzyme-linked immunosorbent assay（ELISA） and compaired with those of healthy control group.Result：Serum IL-4 levels were significantly higher in the JORRP children（P〈0.01）：（524.65±147.77）pg/ml in the JORRP children and（213.27±87.48）pg/ml in the healthy control group.Serum IFN-γ levels were significantly lower in the JORRP children（P〈0.01）：（2.87±0.84）pg/ml in the JORRP children and（10.63±5.09）pg/ml in the heathy control group.Serum IL-32 levels were significantly lower in the JORRP children（P〈0.01）：（2.47±1.60） pg/ml in the JORRP children and（9.08±2.66）pg/ml in the healthy control group.Conclusion：①While the concentration of Th2 like cytokine IL-4 in children with JORRP was higher than that in control group,the concentration of Th1 like cytokine IFN-γ in children with JORRP was lower than that in controls,indicating that the polarization of Th1 /Th2 T cell in children with JORRP;②The polarization of Th1 /Th2 T cell may cause the reduction of the serum IL-32 as a proinflammatory role in host immunity system that could not eradicate HPVs because of lacking enough inflammatory stimulation.

关 键 词：幼年复发性呼吸道乳头状瘤 白细胞介素-4 干扰素-Γ 白细胞介素-32 血清 免疫 

分 类 号：R739.65[医药卫生—肿瘤]