大鼠局灶性脑缺血再灌注TTC染色与MRI弥散加权成像的相关性研究  

Correlation of MR diffusion imaging and triphenyl tetrazolium chloride staining in rats after focal cerebral ischemia and reperfusion

作　　者：吴文娟[1] 王成达[2] 于向荣[1] 

机构地区：[1]无锡市第二人民医院影像科,214002 [2]无锡市中医医院影像科,214001

出　　处：《中华神经医学杂志》2013年第6期561-564,共4页Chinese Journal of Neuromedicine

摘　　要：目的研究大鼠局灶性脑缺血再灌注MRJ弥散加权成像（DWI）的变化，探讨其与TTC染色变化的关系。方法44只雄性SD大鼠按随机数字表法分为假手术组和脑缺血2h、脑缺血6h再灌注不同时间组（0h、0．5h、2h、6h、24h），每组各4只。脑缺血2h组、脑缺血6h组采用线栓法制作成大脑中动脉栓塞模型。各组大鼠于不同再灌注时间点行MRIDWI扫描，结束后立即处死，取相应部位脑组织行TTC染色，观察分析其与DWI成像的关系。结果脑缺血2h组、脑缺血6h组再灌注0h均可见部分右侧大脑半球DWI呈高信号，表观扩散系数（ADC）值降低，TTC染色可见部分右侧大脑半球失染呈白色。缺血2h组再灌注24h后DM异常信号区相对面积明显变小，ADC值增大，TTC失染相对面积变小，较再灌注0h差异有统计学意SL（P〈0．05），而脑缺血6h组则差异无统计学意义（P〉0．05）。脑缺血6h组再灌注不同时间点较脑缺血2h组再灌注相应时间点DWI异常信号区相对面积大，ADC值低，TTC失染相对面积大，差异有统计学意义（P〈0．05）。脑缺血2h组、脑缺血6h组再灌注相对应时间点DWI异常信号区面积与TTC染色失染区面积比较差异无统计学意义（p〉0．05）。结论脑缺血再灌注后可用MRIDWI成像评价脑缺血面积。Objective To investigate the changes of MR diffusion imaging （DWI） appearance in rats after focal cerebral ischemia reperfusion, and explore its relation with the changes of triphenyl tetrazolium chloride （TTC） staining. Methods Forty-four male Sprague Dawley rats were randomly divided into a sham-operated group （n=4） and two experimental groups （n=20, ischemia for 2 and 6 h, respectively）; rats in the experimental groups were performed occlusion of the right middle cerebral artery （MCAO） with thread, and reperfusion for 0, 0.5, 2, 6 and 24 h （n=4）. DWI was performed at each time point; and then, the rats were sacrificed and TTC staining was performed. Results Before reperfusion, DWI demonstrated hyperintensity in the right hemisphere in the experimental groups, the ADC value was decreased, and white area was noted by TTC staining. The experimental group of ischemia for 6 h had larger area of DWI abnormal signal and decreased apparent diffusion cofficient （ADC） value as compared with those in the experimental group of ischemia for 2 h （P〈0.05）. In experimental group of ischemia for 2 h, area of DWI abnormal signal shrank 24 h after reperfusion （P〈0.05）, but that was slightly diminished in experimental group of ischemia for 6 h. No marked difference of abnormal area was noted between those shown by DWI and TTC staining （P〉0.05）. Conclusion DWI can reflect the infarction area in rats after focal cerebral ischemia reperfusion.

关 键 词：脑缺血再灌注 磁共振成像 弥散加权成像 TTC染色 

分 类 号：R743.3[医药卫生—神经病学与精神病学]