不同浓度组织蛋白酶抑制剂E-64对牙本质与树脂粘接耐久性的影响  

Effect of different molarity cathepsins specific inhibitor E-64 on dentin-resin bonding durability

作　　者：杨伟湘[1] 张文浩[1] 吴淑仪[1] 姚科[1] 梁国斌[1] 李彦[1] 

机构地区：[1]中山大学光华口腔医学院·附属口腔医院口腔修复科·广东省口腔医学重点实验室,广州510055

出　　处：《中华口腔医学杂志》2013年第6期368-371,共4页Chinese Journal of Stomatology

基　　金：广东省科技计划（2011B061300088）

摘　　要：目的探讨组织蛋白酶特异性抑制剂N-（反式-环氧丁二酰基）-L-亮氨酸-4-胍基丁基酰胺[N-（trans—epoxysuceinyl）-L-leueine 4-guanidinobutylalllide，E-64]对牙本质与树脂粘接耐久性的影响及其提高粘接耐久性的最佳有效浓度，为临床使用E-64提高牙本质与树脂的粘接耐久性提供实验依据。方法50颗人第三磨牙按随机数字表法随机分为5组，每组10颗，分别用浓度为0（空白对照组）、2．5、5．0、10．0和20．0μmol／LE-64处理牙本质粘接界面。每组制作20个微拉伸试件，其中10个置于37℃水储存24h（老化处理前），另10个于37℃水储存3个月并行冷热循环3000次（老化处理后），分别测试各组试件老化处理前后的微拉伸强度（即粘接强度）。扫描电镜观察试件断裂界面并分析断裂类型。结果各组试件老化处理前的粘接强度差异无统计学意义（P〉0．05）。而老化处理后，2．5、5．0、10．0、20．0μmol／L组试件粘接强度分别为（18．7±2．7）、（20．8±3．4）、（18．3±2．8）和（19．1±2．7）MPa，均高于空白对照组[（15．1±3．0）MPa]，差异有统计学意义（P〈0．05）。除5．0μmlol／L组老化处理前后粘接强度差异无统计学意义外（P〉0．05），其他各组试件老化处理后的粘接强度均显著低于老化处理前（P〈0．05）。扫描电镜观察显示老化处理后各组试件断裂界面破坏模式以界面破坏与混合破坏为主，各E-64组胶原纤维网均较空白对照组完整。结论组织蛋白酶抑制剂E-64处理牙本质粘接界面可有效抑制牙本质与树脂粘接界面胶原纤维网的老化降解，提高牙本质与树脂的粘接耐久性。Objective To investigate, in vitro, the effect of cathepsins specific inhibitor N-（ trans- epoxysuccinyl）-L-leucine 4-guanidinobutylamide （E-64） on dental endogenous cathepsins and to find its most effeclive molarity to elevate dentin-resin bonding durability. Methods Fifty recently extracted human third molars were divided into five groups according to random number table, and treated with different molarity of E-64 as follow： 0, 2. 5, 5. 0, 10. 0 and 20. 0 wnol/L The group 0 μmol/L was control group. Then 20 specimens of dentin-resin composite were fabricated in each group. Half of the specimens were tested after 24 h water storage（ 37 ℃ ） and the other half were tested after 90 days water storage （37 ℃ ） [bllowed by 3000 cycles＇thermocyling（5-55 ℃ ） as aging treatment. Fractured specimens were analyzed using scanning electron lnicroscopy （ SEM ）. Results After 24 h water storage, no significant differences were found in micro-tensile bond strength（μTBS） of samples between different groups （P 〉 0. 05）. However, after ageing trealment, t,TBS of the samples in group 2. 5, 5.0, 10. 0 and 20. 0 μmol/L [ （ 18.7 ± 2.7）, （20. 8 ± 3.4）, （18.3 ± 2.8） and （19. 1 ± 2.7） MPal were significantly higher than that in group 0 μmol/L [ （ 15. 1 ±3.0） MPal （ P 〈 0. 05 ）. Only in the group 5.0 μmol/L no significant difference was found between the original and the decreased value of laTBS（ P 〉 0. 05 ） , while the μTBS in other groups decreased significantly after aging treatment（ P 〈0. 05 ）. Failure types were almost adhesive and mixed types. Collagens in hybrid layer were less degraded in the groups using E-64 after aging treatment than control group.Conclusions E-64 was effective on inhibiting cathepsins activity in dentin, and induced less collagens degradation in smear layer for better dentin-resin bond durability.

关 键 词：组织蛋白酶类 酶抑制剂 牙本质粘结剂 

分 类 号：R783.1[医药卫生—口腔医学]