δ阿片受体激动剂DADLE对急性全脑缺血再灌注大鼠脑组织Bcl-2、Caspase-3的影响  

Effects of DADLE on Bcl-2 and Caspase-3 of rats’ brain expression following cerebral- ischemia reperfusion injury

作　　者：文锐玲[1] 郭伟[1] 黄伟青[1] 武钊[1] 刘佩仪[1] 洪妙玲[1] 郭兰英[1] 黄瑞娇[1] 欧阳斌[1] 梁庆[1] 

机构地区：[1]广州医学院第一附属医院,510120

出　　处：《中国医疗前沿》2013年第9期21-23,共3页China Healthcare Innovation

基　　金：2011年广州市大学生科技创新实践项目(编号:1057011004);广州市医药卫生科技项目(编号:201102A213152)

摘　　要：目的研究全脑缺血再灌注给予δ阿片受体激动剂DADLE前后大鼠脑组织Bcl-2、Caspase-3的表达情况,探讨DADLE在全脑缺血再灌注损伤中对Bcl-2、Caspase-3的影响及与脑保护作用的关系。方法健康雌性SD大鼠50只(180-220g)随机分为5组:假手术组(Sham,n=10):只暴露血管而不夹闭;缺血再灌注组(I/R,n=10):采用改良的二血管阻断加低血压法建立全脑缺血再灌注模型,缺血15min后给予再灌注;DADLE处理组分为2mg/kg组(A,n=10)、DADLE 3mg/kg组(B,n=10)、DADLE 5mg/kg组(C,n=10):在I/R组的基础上于再灌注前经颈外静脉注射DADLE。各组动物再灌注120min后处死,开颅取大脑组织,采用western-bolt技术检测大鼠脑组织Bcl-2、Caspase-3的表达状况。结果 western-bolt检测结果显示,sham组Bcl-2表达水平显著低于其他各组(P<0.05),且Sham组仅少量Caspase-3表达。DADLE处理组与I/R组相比,Bcl-2表达水平上调(P<0.05)而Caspase-3表达水平下调(P<0.05)。各给药组之间Bcl-2为B组与C组的表达显著高于A组(P<0.05),B组与C组间差异无统计学意义(P>0.05);Caspase-3表达水平为B组与C组的表达显著低于A组(P<0.05),而B组与C组间差异无统计学意义(P>0.05)。结论药物DADLE可能通过上调Bcl-2的表达并抑制Caspase-3的表达,抑制神经细胞的凋亡,减低全脑缺血再灌注损伤,从而对大脑起保护作用。Objective To determine expression of Bcl-2 and Caspase-3 in brain tissue of rat before and after using DADLE, and to investigate effects of DADLE on Bcl-2 and Caspase-3 expression following global cerebral- ischemia reperfusion injury and the relationship with Brain protection. Methods 50 Sprague-Daw-ley（SD） rats were randomly entolled into 5 groups., the sham-operated： only exposure blood vessels, the ischemia reperfusion group（I/R, n=10）： improved establishment of two vessel occlusion add hypotensionand establish global cerebral-ischemia reperfusion, 15rain after ischemia and give reperfusion, three DADLE-treated（different drug doses： 2mg/kg[A], 3mg/kg[B] and 5mg/kg[C]）： via external jugular vein injection of DADLE before reperfusion on the basis of the I/R group. Each animal Put to death after reperfusion for 120rain, Craniotomy for brain tissue, Using western-bolt technique to detect the expression of Caspase-3 and Bcl-2 in brain tissue of rat. Results Western-bolt test results： The expression level of Bcl-2 in sham group was significantly lower than that of other groups（P〈0.05）, and only a small amount of Caspase-3 expression in Sham group. DADLE group compared with I/R group, Bcl-2 expression level increased（P〈0.05） and Caspase-3 expression level decreased（P〈0.05）. Each group of Bcl-2 between B group and C group expression was higher than that in A group（P〈0.05）, 13 group and C group had no difference（P〉0.05）. the expression level of CaspaseT-3 in B group and C group was significantly lower than that in group A expression（P〈0.05）, 13 ;group and C group differences no statistical significance（P〉0.05）. Conclusion Substance DADLE may through the up-regulation of Bcl-2 expression and inhibit the expression of Caspase-3, inhibit the apoptosis of nerve cells, reduce the total cerebral ischemia reperfusion injury, which helps protect the brain.

关 键 词：全脑缺血再灌注 DADLE 神经保护 BCL-2 CASPASE-3 

分 类 号：R364.12[医药卫生—病理学]