异叶青兰总黄酮对高血压大鼠心肌肥厚的影响  

Effects of Dracocephalum heterophyllum Benth flavonoid on cardiac hypertrophy of hypertension rats

作　　者：何雯[1,2] 邬利娅.伊明 司丽君[1] 苗娜[3] 阿吉艾克拜尔.艾萨 帕尔哈提.克热木 

机构地区：[1]新疆医科大学基础医学院药理学教研室,新疆乌鲁木齐830054 [2]新疆医科大学第五附属医院药剂科,新疆乌鲁木齐830054 [3]新疆医科大学第一附属医院病理科,新疆乌鲁木齐830054 [4]中国科学院新疆理化技术研究所,新疆乌鲁木齐830011

出　　处：《中国药理学通报》2013年第7期1008-1011,共4页Chinese Pharmacological Bulletin

基　　金：省部共建国家重点实验室培育基地新疆特有药用资源利用实验室开放课题项目(No XJYS0204-2012-01);新疆维吾尔自治区自然科学基金项目(No 2012211B09);新疆医学动物模型研究重点实验室开放课题项目(NoXJDX1103-2011-03)

摘　　要：目的观察异叶青兰总黄酮对肾性高血压大鼠心肌肥厚的影响。方法左肾动脉狭窄(2K1C)法建立高血压大鼠模型,术后第6周随机分为5组:假手术组(Sham);模型组(Model);异叶青兰总黄酮高剂量组(DHBFH)、低剂量组(DHBFL);卡托普利组(Captopril)。灌胃给药6周后进行超声心动图、心肌病理学检测,白介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)、基质金属蛋白酶-9(MMP-9)和基质金属蛋白酶抑制剂-1(TIMP-1)mRNA的测定。结果给药6周后,模型组大鼠左心室室壁厚度、心肌细胞大小及左心室心肌间质纤维化程度较假手术组明显升高,IL-1β、TNF-α明显升高(P<0.01),MMP-9、TIMP-1 mRNA的相对表达量明显升高(P<0.01),给予DHBFH后,左心室肥厚及心肌纤维化较模型组明显降低,IL-1β、TNF-α明显降低,MMP-9、TIMP-1 mRNA的相对表达量明显降低(P<0.01)。结论异叶青兰总黄酮能改善高血压大鼠心肌肥厚及心肌纤维化程度,可能与其能够降低血压,降低IL-1β、TNF-α水平,调节MMP-9/TIMP-1的表达有关。Aim To study the effect of Dracocephalum heterophyllum Benth flavonoid （DHBF） on cardiac hy- pertrophy of hypertension rats. Methods Hyperten- sion rats model was established by renal artery stenosis surgery （two kidneys one clip）. Six weeks after the surgery, the rats were randomly divided into 5 groups Sham group; Model group; DHBF high dose group （DHBFH） ; DHBF low dose group（DHBFL） and Cap- topril group. After treated for 6 weeks, the echocardio- graphy, histological examination of the heart were per- formed. The levels of IL-1β, TNF-α, the relative ex- pression of MMP-9 mRNA and TIMP-1 mRNA were de- tected by real-time PCR . Results After 6 weeks treatment, the wall thickness of left ventricle, myocar- dial cell size and interstitial fibrosis of the myocardial cells in the left ventricl in Model group were signifi-cantly increased compared with those in sham group （P 〈0. O1 ）. Treatment with DHBFH for 6 weeks signifi- cantly attenuated left ventricular hypertrophy （P 〈 0.01 ）. Biochemical testing revealed ,that the levels of IL-1β,TNF-α, the expression of MMP-9 and TIMP-1 reduced after treatment with DHBF, the levels of them were significantly increased in model group （P 〈 0.01 ）. Conclusions The results suggest that DHBFH treatment significantly inhibits the cardiac hypertrophy and interstitial fibrosis in hypertension rats. Its possible mechanisms are via lowering blood pressure, reducing the level of IL-1β ,TNF-α and balancing the expression of MMP-9/TIMP-1.

关 键 词：异叶青兰总黄酮 高血压 心肌肥厚 白介素-1Β 肿瘤坏死因子-α 基质金属蛋白酶-9 基质金属蛋白酶抑制剂-1 

分 类 号：R-332[医药卫生] R284.1