5-氮杂-2'-脱氧胞苷与顺铂对MDA-MB-231细胞的联合作用  

Effect of combining treatment of 5-Aza-CdR and cisplatin on MDA-MB-231 cells

作　　者：沈三弟[1] 陈卓荣[1] 肖高芳[2] 刘彦明[3] 

机构地区：[1]粤北人民医院头颈乳腺外科,广东韶关512026 [2]粤北人民医院病理科 [3]粤北人民医院检验科

出　　处：《中国肿瘤外科杂志》2013年第3期176-178,共3页Chinese Journal of Surgical Oncology

基　　金：韶关市科技计划项目(No.2012CX/K72)

摘　　要：目的探讨5-氮杂-2'-脱氧胞苷(5-Aza-CdR,DAC)与顺铂(cisplatin,PDD)联合应用对人三阴性乳腺癌细胞株MDA-MB-231体外增殖及凋亡的影响。方法实验分组:5μM DAC处理组(DAC组),15μM PDD处理组(PDD组),2.5μM DAC与8μM PDD同步处理组(DAC+PDD组),2.5μM DAC与8μM PDD序贯处理组(DAC→PDD组)及空白对照组。分别以MTT法和流式细胞术(FCM)测定各处理组MDA-MB-231细胞的增殖、凋亡情况,以q值评价两药的联合效应。结果 DAC组的24h、48h、72h增殖抑制率分别为(8.12±0.79)%、(21.72±1.60)%及(30.39±1.31)%;PDD组为(35.14±2.00)%、(49.22±1.01)%及(65.52±1.53)%;DAC+PDD组为(54.25±3.82)%、(68.89±1.52)%及(87.26±2.37)%;DAC→PDD组为(6.84±0.68)%、(67.64±0.91)%及(88.76±3.54)%。联合组较单药组增殖抑制率均显著升高(P<0.01)。DAC+PDD组、DAC→PDD组24h、48h、72h的q值分别为1.12、1.14、1.15和0、1.12、1.17,两药联合有增效作用。对照组、DAC组、PDD组、DAC+PDD组、DAC→PDD组24h、48h、72h凋亡率分别为(1.57±0.38)%、(1.83±0.27)%、(2.26±0.42)%;(10.41±0.70)%、(15.37±0.74)%、(21.39±1.22)%;(16.63±0.65)%、(21.89±1.20)%、(30.39±2.20)%;(21.42±1.11)%、(33.86±1.16)%、(42.92±1.16)%;(8.26±0.68)%、(28.98±1.01)%、(41.98±1.12)%。联合组较单药组及对照组凋亡率显著升高(P<0.01)。结论 DAC与PDD均能抑制MDA-MB-231细胞株的增殖,促进其凋亡,且两者联合有增效作用。Objective To evaluate the effect of combining treatment of 5-Aza-CdR （DAC） and cisplatin （PDD） on triple-negative breast cancer （TNBC） cell line-MDA-MB-231 in vitro. Methods The investigation was divided into five groups： DAC（5 μM）, PDD（15 μM）, simultaneous DAC 2.5 μM and PDD 8 μM（DAC ± PDD）, PDD 8 μM fellowing DAC 2.5 μM（ DAC→,PDD）, control. Cells proliferation inhibition rate（ PIR）, cells apoptosis rate（AR） and combining effect were measure with MTT, FCM and Jins Formula respectively. Result The PIR of DAC, PDD, DAC ± PDD and DAC→PDD groups were （8. 12 ± 0. 79） %, （21.72 ± 1.60）%, （30.39±1.31）%; （35.14±2.00）%, （49.22±1.01）%, （65.52±1.53）%; （54.25±3.82）%, （68.89±1.52）%, （87.26 ±2.37）%; （6.84 ^-0.68）%, （67.64 ±0.91）%, （88.76± 3.54） % at 24 h, 48 h, 72 h. The PIR of combining groups were higher than that of single-agent groups（P 〈 0.01 ） and there were no significant difference between combining groups at 72 h（P =0. 174）. The q value of DAC ±PDD and DAC--~PDD groups were 1.12, 1.14, 1.15;0, 1.12, 1.17 at24 h, 48h, 72h. The synergis- tic effect existed in combining groups. The AR of control, DAC, PDD, DAC ± PDD and DAC---~PDD groups were（1.57±0.38）%, （1.83±0.27）%, （2.26±0.42）%; （10.41 ±0.70）%, （15.37±0.74）%, （21.39 ±1.22）%; （16.63±0.65）%, （21.89±1.20）%, （30.39 ±2.20）%; （21.42 ±1.11）%, （33.86±1.16）%, （42.92±1.16）%; （8.26±0.68）%, （28.98±1.01）%, （41.98±1.12）% at24 h, 48 h, 72 h. The AR of combining groups were higher than that of single-agent groups （P 〈 0.01 ） and there were no significant difference between combining groups at 72 h （P = 0. 139 ）. Coneluslons Both DAC and PDD can inhibit MDA-MB-231 cells proliferating and hasten them apoptosis. The combination of DAC and PDD brought synergistic effect.

关 键 词：三阴性乳腺癌 5-氮杂-2'-脱氧胞苷 顺铂 序贯治疗 同步治疗 增殖 凋亡 

分 类 号：R737.9[医药卫生—肿瘤]