β-catenin、cyclinD1、C-myc在儿童肾母细胞瘤中的表达及临床意义  

The Expression and clinical significance of β-catenin,cyclinD1 and C-myc in childhood nephroblastoma

作　　者：杨静薇[1] 蒋慧[1] 王雪莉[1] 史青[1] 陆正华[1] 邵静波[1] 李红[1] 张娜[1] 

机构地区：[1]上海交通大学附属儿童医院血液肿瘤科,上海200040

出　　处：《中国小儿血液与肿瘤杂志》2013年第3期116-119,共4页Journal of China Pediatric Blood and Cancer

摘　　要：目的探讨Wnt信号通路中β-catenin、cyclinD1和C-myc蛋白在儿童肾母细胞瘤中的表达及临床意义。方法用免疫组织化学EnVision System二步法检测34例初发小儿肾母细胞瘤及15例肾母细胞瘤旁正常肾组织病理石蜡标本中β-catenin、CyclinD1和C-myc蛋白的表达情况。结果儿童肾母细胞瘤中β-catenin、cyclinD1和C-myc表达阳性率高于瘤旁正常肾脏组织,差异有显著性,三者表达的阳性率呈正相关性(P<0.01);β-catenin、cyclinD1及C-myc的表达分别与儿童肾母细胞瘤组织分型、临床分期或复发转移相关(P<0.05)。结论β-catenin、cyclinD1和C-myc在儿童肾母细胞瘤组织中表达阳性率高,联合检测β-catenin、cyclinD1和C-myc有助于判断预后。Objective To investigate the expression and the clinical significance of β-catenin, cyclinD1 and C-myc in nephroblastoma. Methods Immunohistochemistry and Envision System were used to investigate the expression levels of β-catenin, cyclinD1 and C-myc protein in 34 pathologic paraffin samples of initial nephroblastoma as well as in 15 normal renal tissues adjacent to the tumor. Results （ 1 ） The expression levels of β-catenin （ P 〈 0. 01 ）, cyclinD1 （ P 〈 0. 01 ） and C-mye （ P 〈 0. 05 ） in nephroblastoma were significant higher than those in normal renal tissues. The expression levels of β-catenin, cyclinD1 and C-myc were positively correlated （P 〈 0. 01 ） . （2） Their expression levels were correlated with the histological types, clinical Stages or relapse and metastasis （P 〈 0. 05）. Conclusions The expression levels of β-catenin, cyclinD1 and C-myc axe dramatically higher in nephroblastoma samples. A combination of measuring β-catenin, cyclinD1 and C-myc contributes to a better evaluation of the prognosis.

关 键 词：肾母细胞瘤 Β-连接素 细胞周期蛋白D1 C—myc蛋白 免疫组织化学 

分 类 号：R737.11[医药卫生—肿瘤]