Effects of morphine on associative memory and locomotor activity in the honeybee (Apis mellifera)  

作　　者：Yu Fu Yanmei Chen Tao Yao Peng Li Yuanye Ma Jianhong Wang 

机构地区：[1]Biomedical Engineering laboratory, Information School, Yunnan University [2]State Key Laboratory of Brain and Cognitive Science, Kunming Institute of Zoology, Chinese Academy of Sciences

出　　处：《Neuroscience Bulletin》2013年第3期270-278,共9页神经科学通报（英文版）

基　　金：supported by grants from the Training Program for Young Backbone Teachers of Yunnan University, the Research Foundation of Yunnan University (2011YB22);the Natural Science Foundation of Yunnan Province, China (2011FZ012);the Special Fund of the "211" Third Phase Project of Yunnan University (21134018);a National Project (2007ZDGGKVST003);a Provincial National Project (2008CA012-5);National Basic Research Development Program (973 program) of China (2012CB825500, 2011CB70780);National Natural Science Foundation of China (31271168);"Strategic Priority Research Program" of the Chinese Acedemy of Sciences, China (XD B02020500)

摘　　要：Morphine can modulate the processes underlying memory in vertebrates. However, studies have shown various modulations by morphine： positive, negative and even neutral. The honeybee is a potential platform for evaluating the effects of drugs, especially addictive drugs, on the nervous system. However, the involvement of morphine in learning and memory in insects or other invertebrates is poorly understood. The current work evaluated whether morphine affects memory acquisition, consolidation and retrieval in honeybees, using the proboscis extension response （PER） paradigm. We demonstrated that morphine treatment （5 pg/bee） before training decreased the percentage of correct PERs and the response latency related to aversive rather than rewarding odors when tested after 1 or 24 h. Morphine treatment after training also caused a decrease in this latency when tested after 24 h. Meanwhile, morphine treatment reduced the ambulation distance when tested after 30 min. Our findings suggest that morphine impairs the acquisition of shortand long-term associative memory and slightly disrupts the consolidation of long-term memory in honeybees. These negative effects cannot be explained by reduced locomotion but by impaired memory associated with aversion.Morphine can modulate the processes underlying memory in vertebrates. However, studies have shown various modulations by morphine： positive, negative and even neutral. The honeybee is a potential platform for evaluating the effects of drugs, especially addictive drugs, on the nervous system. However, the involvement of morphine in learning and memory in insects or other invertebrates is poorly understood. The current work evaluated whether morphine affects memory acquisition, consolidation and retrieval in honeybees, using the proboscis extension response （PER） paradigm. We demonstrated that morphine treatment （5 pg/bee） before training decreased the percentage of correct PERs and the response latency related to aversive rather than rewarding odors when tested after 1 or 24 h. Morphine treatment after training also caused a decrease in this latency when tested after 24 h. Meanwhile, morphine treatment reduced the ambulation distance when tested after 30 min. Our findings suggest that morphine impairs the acquisition of shortand long-term associative memory and slightly disrupts the consolidation of long-term memory in honeybees. These negative effects cannot be explained by reduced locomotion but by impaired memory associated with aversion.

关 键 词：MORPHINE MEMORY locomotor activityhoneybee 

分 类 号：Q965[生物学—昆虫学]