5-氮杂-2-脱氧胞苷提高结肠癌SW620细胞株对5-FU的化学敏感性  

5-aza-CdR enhances chemosensitivity of colon cancer SW620 cells to 5-FU

作　　者：余小燕[1] 吴莺[1] 蒋双红[1] 王静[1] 徐岷[1] 周红[1] 

机构地区：[1]江苏大学附属医院消化病科,212001

出　　处：《江苏医药》2013年第11期1259-1262,F0002,共5页Jiangsu Medical Journal

基　　金：江苏省自然科学基金(BK2010336)

摘　　要：目的探讨5-氮杂-2-脱氧胞苷(5-aza-CdR)可能增强结肠癌SW620细胞株对5-氟尿嘧啶(5-FU)化学敏感性的作用。方法分别用不含药物组(A组)、5-aza-CdR(B组)、5-FU(C组)以及5-aza-CdR联合5-FU(D组)作用人结肠癌SW620细胞株后,光镜下观察细胞形态学改变,MTT法测定细胞的生长情况和流式细胞术检测细胞凋亡。结果 A、B、C、D组细胞存活率分别为100%、(65.85±0.95)%、(45.14±1.44)%、(30.18±0.89)%;B、C、D组细胞存活率均明显低于A组(P<0.05),D组低于B、C组(P<0.05)。A、B、C、D组细胞凋亡率分别为(4.50±0.61)%、(16.80±0.57)%、(12.66±0.43)%、(42.07±0.85)%;与A组比较,B、C、D组引起细胞凋亡增加(P<0.05);与B组、C组比较,D组促进细胞凋亡作用更明显(P<0.05)。结论 5-aza-CdR在体外提高了结肠癌SW620细胞对5-FU的化学敏感性。Objective To investigate the potential effect of 5-aza-2-deoxycytidine（5-aza-CdR） on enhancing the chemosensitivity of colon cancer SW620 cells to 5-FU. Methods The SW620 cells were treated with 5-aza-CdR（group B）, 5-FU（group C）, 5-aza-CdR combined with 5-FU（group D） and without drug（group A as blank control）, respectively. The morphology of the cells was observed under inverted phase contrast microscope. Cell proliferation was evaluated by MTT assay. Flow cytometry was used to examine the apoptosis rate. Results The cell viabilities of groups of B,C and D were （ 65.85 ± 0.95） %, （45.14 ±1.44） % and （ 30. 18 ±0. 89 ）%, respectively, which were significantly lower than 100% of group A（P〈0. 05）. Compared with groups of B and C, the reduction of cell viability in group D was more（P〈0. 05）. The apoptosis rates of groups B,C and D were （16.80±0. 57） %, （12.66±0. 43） % and （42.07± 0. 85） %, respectively , which were obviously higher than （4. 50±0.61）%of group A（P〈0. 05）. Compared with groups of B and C, cell apoptosis rate of group D was more （P〈0. 05）. Conclusion 5-aza-CdR in vitro enhances the chemosensitivity of 5-FU in colon cancer SW620 cells.

关 键 词：5-氮杂-2-脱氧胞苷 结肠癌SW620细胞 5-氟尿嘧啶 

分 类 号：R735[医药卫生—肿瘤]