RNAi沉默Stat1基因对胶质瘤细胞FAT10表达的影响  

Effects of silencing Stat1 by siRNA on the expression of FAT10 protein in glioma cells

作　　者：李少一[1] 王志超[2] 高芸[3] 李晓东[1] 张明杰[1] 马维宁[1] 刘云会[1] 

机构地区：[1]中国医科大学附属盛京医院神经外科,辽宁沈阳110041 [2]齐齐哈尔医学院期刊社 [3]中国医科大学附属第一医院内分泌研究所

出　　处：《中国老年学杂志》2013年第11期2564-2566,共3页Chinese Journal of Gerontology

基　　金：国家自然科学基金项目(No.81102471);辽宁省科学技术基金(No.201202286);辽宁省教育厅科学研究项目(No.L2010586);盛京自由研究者基金

摘　　要：目的观察U251人胶质瘤细胞中Stat1基因沉默对FAT10蛋白表达的影响。方法采用电穿孔法将Stat1siRNA转染人胶质瘤细胞系U251细胞,采用Western印迹检测各组细胞中Stat1和FAT10蛋白的表达变化,用染色体核型分析Stat1干扰对FAT10过表达而导致的非整倍体现象的阻断作用。结果 Western印迹结果显示,Stat1 siRNA干扰可以有效抑制U251细胞中Stat1的表达,并且通过抑制Stat1可以有效抑制TNF-α诱导的FAT10高表达,进而通过有效抑制TNF-α诱导的FAT10高表达而阻断TNF-α诱导的非整倍体现象。结论 Stat1的表达下调可以抑制TNF-α诱导的FAT10高表达及非整倍体现象。Objective To observe the FAT10 protein expression in U251 cells after Statl gene silence induced by siRNA. Methods The electroporation System was used to transfect siRNA into U251 cells . Western blot was employed to detect the levels of FATIO and Statl proteins in U251 cells after transfection. Cytogenetie analysis was performed to analyze the chromosome numbers. Results The TNF- ct induced over-expression of FAT10 gene was significantly inhibited after Statl siRNA transfection. At the same time, TNF-ct induced abnor- mal chromosome karyotype were also blocked. Conclusions siRNA interference targeting Statl might inhibit the expression of FAT10 gene and TNF-ct induced abnormal chromosome karyotype in U251 cells.

关 键 词：小干扰RNA STAT1 FAT10 胶质瘤 

分 类 号：R739.41[医药卫生—肿瘤]