天麻通脑颗粒对急性脑梗死大鼠血管神经再生的影响及作用机制  

Action mechanism of Gastrodia Tongnao Particles on vascular nerve regeneration of acute cerebral infarction rats

作　　者：王国媛[1] 吴明华[1] 韩漾[2] 梁森[2] 马立[2] 吴颢昕[1] 

机构地区：[1]南京中医药大学,江苏南京210009 [2]江苏省中医院神经内科

出　　处：《中国老年学杂志》2013年第11期2566-2568,共3页Chinese Journal of Gerontology

基　　金：江苏省中医药领军人才培养项目(No.LJ200912);江苏省中医药管理局(No.LZ11036);江苏省“六大人才高峰”(No.2011-WS-046)

摘　　要：目的探讨天麻通脑颗粒对大脑中动脉梗死大鼠血管神经再生的作用机制。方法取成年雄性SD大鼠54只,随机分为假手术组(n=18)和模型组(n=36)。模型组大鼠采用线拴法制作大鼠大脑中动脉局灶性缺血再灌注模型,术后随机分为模型对照组(n=18)和给药组(n=18),于24 h后分别通过腹腔注射给予等量等渗生理盐水或天麻通脑颗粒,4 ml/次,2次/d,共14 d。术后2 h、1 d、7 d、14 d四个不同时间点对脑梗死后大鼠神经功能恢复程度进行神经功能缺损评分。采用苏木素-伊红染色法对脑梗死大鼠于术后第14天进行梗死灶体积测定。采用免疫组化法于术后第14天对缺血侧室管膜下区(SVZ)及纹状体进行5-溴脱氧尿核苷(BrdU)阳性细胞计数。结果与模型组相比,给药组从术后第7天开始大鼠神经功能就有显著恢复(P=0.043,P=0.031)。模型对照组与给药组大鼠的梗死灶体积差异有统计学意义(P=0.044)。BrdU免疫组化显示,术后第14天给药组在SVZ及纹状体区域的BrdU阳性细胞数量较模型对照组有显著增高(P=0.009,P=0.044)。结论天麻通脑颗粒可以促进脑缺血大鼠神经功能的恢复,对缺血后脑细胞的增殖具有一定疗效。Objective To investigate the mechanism of Gastrodia Tongnao Particles on the cerebral vascular nerve regeneration after cerebral infarction in middle cerebral artery occlusion （MCAO） rats. Methods 54 adult male SD rats were randomly divided into sham group （n = 18） and model group （n = 36）. Model group rats established by MCAO were randomly divided into control group （n = 18） and dose group （n = 18）, respectively. After 24 h, control group were injected the same amount of isotonic saline by intraperitoneal, and dose group were injected Gastrodia Tongnao Particles by intraperitoneal（4 ml/time, 2 times/d, a total of 14 days）. Neurological deficits were evaluated at 2 h, day 1, day 7, day 14, and infarct volume were assessed by hematoxylin and eosin-stained brain sections at day 14 in rats. Cells staining by Brdu were counted as immunopositive stained cells （Brdu） from ischemic subependymal zone （SVZ） and striatum in day 14. Results Compared with control group, dose group had a significant recovery of neurologic function from 7 days in rats（ P = 0. 043, P = 0. 031 ）. Gastrodin Tongnao Particle had significantly improved the neurologic functions in rats. The infarct volume of dose group rats was significantly different compared with that of control group（ P =0. 044）. BrdU positive ceils in the SVZ and striatum area of treatment group was significantly higher compared with the control group at day 14 （P = 0. 009,P = 0. 040）. Conclusions Gastrodia Tongnao Particles can promote the recovery of neurological function, and has an effect on the proliferation of brain cells after ischemic infarction in MCAO rats.

关 键 词：天麻通脑颗粒 脑梗死 血管神经再生 大脑中动脉局灶性缺血再灌注(MCAO) 

分 类 号：R743[医药卫生—神经病学与精神病学]