曲古抑菌素A抑制乳腺癌细胞MCF-7生长增殖及促进凋亡的机制  

The mechanism of TSA inhibiting proliferation and promoting apoptosis in breast carcinoma cell line MCF-7

作　　者：张秀梅[1] 刘超[1] 刘阳[1] 肖建英[1] 

机构地区：[1]辽宁医学院生物化学教研室,辽宁锦州121000

出　　处：《中国老年学杂志》2013年第11期2569-2572,共4页Chinese Journal of Gerontology

基　　金：辽宁省教育厅重点实验室项目(LS2010101)

摘　　要：目的探讨曲古抑菌素A(TSA)对乳腺癌细胞系MCF-7细胞生长增殖、凋亡及p21、p53表达的影响。方法分别以不同浓度的TSA处理MCF-7细胞,采用MTT比色法测定细胞增殖活性;用流式细胞仪检测细胞凋亡率;用RT-PCR方法检测细胞p21、p53mRNA的表达水平;用Western印迹法检测MCF-7细胞的p21、p53蛋白表达。结果 TSA作用后,各组细胞均出现显著的生长抑制作用,存活率明显降低,并呈剂量依赖性。流式细胞仪分析,在100～400 nmol/L范围内凋亡率随TSA浓度的升高而升高(P<0.01)。经不同浓度TSA处理的细胞p21mRNA和蛋白表达水平明显上调(P<0.01);、而p53mRNA和蛋白表达水平则没有明显变化。结论 TSA显著抑制MCF-7细胞生长,促进凋亡可能与TSA维持p53的稳定表达,从而促进其下游因子p21的表达有关。Objective To study the effects of trichostatin A （TSA） on cell proliferation, apoptosis and expressions of p21, p53 in MCF-7 cells. Methods The proliferation of the cells treated with different dose of TSA were observed with MTT and apoptosis were meas- ured by flow cytometry; the p21, p53 mRNA expression levels were determined by RT-PCR; p21, p53 protein expression were determined by Western blot. Results After cells were treated with TSA the proliferation index decreased markedly with a dose-dependent manner. Flow eytometry analysis showed TSA increased apoptosis index, the mRNA and protein expression of p21 were increased dramatically （ P 〈 O. O1 ） ; but the mRNA and protein expression of p53 were not increased dramatically （P 〉0. 05）. Conclusions TSA could inhibit the preliferation and promote apoptosis of MCF-7 cell in a dose-dependent manner thus up-regulating of p21.

关 键 词：曲古抑菌素A MCF-7 凋亡 P21 p53 

分 类 号：R736.1[医药卫生—肿瘤]