血管内皮细胞增殖过程中碘离子与血管内皮生长因子及其受体的关系  

The relationship between iodide ion and vascular endothelial growth factor together with its receptor in vascular endothelial cell proliferation

作　　者：滕飞[1] 祖茂衡[1] 华浅近[1] 王磊[1] 

机构地区：[1]徐州医学院附属医院介入科,221006

出　　处：《介入放射学杂志》2013年第6期486-489,共4页Journal of Interventional Radiology

基　　金：国家自然科学基金主任基金资助项目(81141074)

摘　　要：目的探讨血管内皮细胞(vascular endothelial cell,VEC)增殖过程中碘离子与血管内皮生长因子(VEGF)及其受体的关系。方法①采用CCK-8法检测碘离子与VEGF抑制剂作用后VEC的增殖率。②采用蛋白质印迹技术(Western印迹法)检测碘离子对血管内皮生长因子受体2(VEGFR-2)磷酸化的影响。结果①在300μg/L碘离子环境中,使用VEGF抑制剂并不能抑制VEC的增殖(P<0.05)。②一定浓度的碘离子可促进VEGFR-2(Tyr1214)磷酸化水平上调(P<0.05)。③碘离子对VEGFR-2(Tyr1175,Tyr951位点)的磷酸化无影响。结论①碘离子促进VEC增殖并不依赖于VEGF对膜受体的刺激,碘离子可视为促VEC增殖的独立因素。②碘离子通过刺激膜受体VEGFR-2(Tyr1214)磷酸化水平上调,介导VEC迁移。③碘离子对VEGFR-2的Tyr1175位点的磷酸化无影响,其对VEC的促增殖效应并不是通过作用于膜受体。Objective To explore the relationship between iodide ion and vascular endothelial growth factor （VEGF） as well as its receptor in vascular endothelial cells （VEC） proliferation. Methods （l） The detection of the proliferation rate of vascular endothelial cell after it reacted to iodide ion and VEGF inhibitor was performed by using CCK-8 kit. （2） The detection of the influence of iodide ion on the phosphorylation of VEGFR-2 was carried out by using Western Blot technique. Results （1） The VEGF inhibitor could not suppress the proliferation of vascular endothelial cell in concentration of 300 ~g/L of iodide ion （P 〈 0.05）. （2） A certain concentration of iodide ion could stimulate the phosphorylation of VEGF-2 （Tyrl214） （P 〈 0.05）. （3） Iodide ion could not affect the phosphorylation of VEGF- 2 （Tyrl175 and Tyr951 loci）. Conclusion （1） Iodide ion can stimulate the proliferation of VEC, and it does not depended on the stimulation of membrane receptor from VEGF. Therefore, iodide ion can be regarded as an independent element in stimulating the proliferation of vascular endothelial cell. （2） The iodide ion can mediate the migration of vascular endothelial cell through stimulating the phosphorylation of VEGF-2 （Tyrl214）. （3） The iodide ion has no effect on the phosphorylation of VEGFR-2 （Tyrl175）. The stimulation of iodide ion to the proliferation of VEC is accomplished not by way of acting on the membrane receptor. （J lntervent Radiol, 2013, 22： 486-489）

关 键 词：布-加综合征 血管内皮细胞 碘离子 实验研究 

分 类 号：R575[医药卫生—消化系统]