^(125)I粒子持续照射对Sw1990及Panc-1细胞生物学效应的影响  

The biological effects of continuous ^(125)I seeds irradiation on sw1990 and panc-1 cells: a comparison study

作　　者：蒋奡[1] 王忠敏[2] 张丽云[2] 茅爱武[3] 刘芬菊[4] 

机构地区：[1]上海交通大学医学院附属仁济医院介入放射科,200127 [2]上海交通大学医学院附属瑞金医院卢湾分院放射科 [3]上海交通大学医学院附属仁济医院长宁分院介入放射科 [4]苏州大学放射医学与防护学院

出　　处：《介入放射学杂志》2013年第6期490-493,共4页Journal of Interventional Radiology

基　　金：国家自然科学基金(81271682);中国博士后科学基金(109312);上海市卢湾区科委医疗卫生科研项目(LKW1104)

摘　　要：目的采用125I粒子对胰腺癌细胞Sw1990及Panc-1行体外持续照射,研究其生物学效应,探讨连续照射对胰腺癌细胞增殖、DNA合成、细胞周期及凋亡的影响,并为胰腺癌放射实验细胞的选择提供参考。方法将胰腺癌细胞Sw1990及Panc-1体外培养至对数生长期,行125I粒子持续照射,在初始剂量率为12.13 cGy/h时,分别给予总剂量为0、2、4、6、8 Gy的照射,采用克隆形成实验检测照射后细胞的增殖能力,绘制生存曲线,计算细胞存活率(SF2),检测细胞凋亡率和细胞周期,以及3H-TDR掺入实验探究照射后细胞DNA合成情况。结果经过拟合,计算出Sw1990和Panc-1细胞的SF2值分别为0.766±0.063和0.729±0.045,随着照射剂量增高,两种细胞凋亡率也逐渐升高,Panc-1细胞的最大凋亡率出现在6 Gy,Sw1990出现在8 Gy。G2/M期阻滞分数均逐渐升高,3H-TDR掺入放射量逐渐降低。结论125I持续照射胰腺癌细胞时,细胞凋亡及G2/M期阻滞是抑制细胞增殖的主要原因,在剂量为0、2、4、6、8 Gy时,Sw1990及Panc-1细胞生物学效应差异无统计学意义。Objective Nowadays the implantation of ^125Ⅰ radioactive seeds into pancreatic cancer is considered to be an effective way to diminish the volume of neoplasm and reduce the pain. This experiment aims to investigate the effects of continuous ^125Ⅰ seeds irradiation in vitro on the proliferation, DNA synthesis, cell cycle and apoptosis of sw1990 and panc- 1 cells of pancreas cancer, so as to provide a useful reference for the selection of experimental cells in doing radiation research of pancreas cancer. Methods Sw1990 and Panc-1 cells in exponential growth were irradiated at an initial dose rate of 12.13 cGy/h in vitro and were kept under exposure until the total dose reached 0, 2, 4, 6 and 8 Gy separately. After irradiation, the cells were collected. The proliferation of ceils after irradiation was evaluated by counting the number of colonies and the cell survival curves were plotted, with which the cell survival rate （SF2） was calculated. The apoptosis of cell, cell cycle and DNA synthesis after the 3H-TDR incorporation experiment were determined. Results The SF2 of SW1990 cells was 0.766 ± 0.063, while the SF2 of Panc- 1 cells was 0.729 ± 0.045. As the dose increasing, the ratio of ^3H-TDR incorporation experiment decreased. The highest rate of apoptosis of Sw1990cells appeared at 8 Gy, while that of Panc- 1 cells appeared at 6 Gy. With the dose increasing, the percentage of GJM cell cycle arrest increased. Conclusion In continuous lzsI seeds irradiation on sw1990 and panc- 1 cells in vitro the major mechanisms of the inhibition of cell proliferation are the apoptosis and the GJ M cell cycle arrest. At the dosage of 0, 2,4, 6 and 8 Gy the biological effects on the Sw1990 cells and on the Panc- 1 cells are of no statistically significant difference. （J Intervent Radiol, 2013, 22. 490-493）

关 键 词：胰腺癌^125Ⅰ粒子 SW1990细胞 Pane-1细胞 

分 类 号：R735.9[医药卫生—肿瘤]