大黄鱼(♀)与鮸状黄姑鱼(♂)杂交及其子代的遗传分析  

The cross breeding and genetic analysis of hybrids of Larimichthys crocea(♀)and Nibea miichthioides(♂)

作　　者：简林江[1] 杨育凯[1] 刘贤德[1] 陈庆凯 王志勇[1] 

机构地区：[1]集美大学水产学院,农业部东海海水健康养殖重点实验室,福建厦门361021 [2]宁德水产技术推广站,福建宁德352100

出　　处：《水产学报》2013年第6期801-808,共8页Journal of Fisheries of China

基　　金：国家"八六三"高技术研究发展计划(2012AA10A403);公益性行业(农业)科研专项(200903046-05);集美大学创新团队科研基金2010A002)

摘　　要：为了探讨大黄鱼(♀)与鮸状黄姑鱼(♂)远缘杂交的可行性,构建了2个杂交家系(LN1和LN2),检测了杂交F1的倍性,并利用10个微卫星标记对杂交亲本及F1进行遗传分析。结果表明,大黄鱼(♀)与鮸状黄姑鱼(♂)可以成功杂交产生形态正常后代,45日龄成活率达到30%,但杂交受精率(29.0%、32.6%)与孵化率(75.0%、76.7%)要显著低于大黄鱼自繁(P<0.05)。杂交幼鱼体型修长,头为尖钝型,体侧布满黑褐色斑点;DNA相对含量测定和微卫星标记分析结果显示,90%以上杂交后代是杂交二倍体,另外有少量的杂交三倍体和雌核发育二倍体;杂交幼鱼形态兼具有双亲的特征,与大黄鱼明显不同;至45日龄为止,杂交二倍体和三倍体生长速度均慢于大黄鱼。研究结果为大黄鱼(♀)和鮸状黄姑鱼(♂)杂交F1的开发利用及管理提供了参考依据。To understand the feasibility of cross breeding between Larimichthys crocea ♀ and Nibea miichthioides ♂,two hybrid families were built（LN1 and LN2）.Ploidy of F1 hybrids was identified.Moreover,ten microsatellite loci were used for genetic relationship analysis between F1 hybrids and their parents.The result showed that L.crocea（♀）and N.miichthioides（♂）can be successfully crossed and hybrid offspring of normal survival were produced.Survival rate of hybrid offspring at the age of 45 days post hatch reached about 30%.However,fertilization rate（29.0%,32.6%）and hatching rate（75.0%,76.7%）of two hybrid families were significantly lower than those in large yellow croaker families（P0.05）.Appearance of juvenile hybrid fish is visibly different from those of large yellow croaker,which has characteristics of both parents：slender body,sharp blunt head and their body sides are covered with black-brown spots.The results of DNA relative content investigation and genetic relationship analysis demonstrated that more than 90% of the hybrid offspring were hybrid diploid,in addition,a small number of those were hybrid triploid and gynogenesis diploid.Till the age of 45 days,growth rate of both hybrid diploid and hybrid triploid was slower than that of large yellow croaker.These findings will provide reference for exploitation and management of F1 hybrids of L.crocea（♀）and N.miichthioides（♂）.

关 键 词：大黄鱼 鮸状黄姑鱼 远缘杂交 DNA相对含量 微卫星标记 遗传关系 

分 类 号：Q321[生物学—遗传学]