HCMV感染及C-Jun表达与结直肠腺癌相关性研究  

Correlation of Human Cytomegalovirus Infection and Expression of C-Jun Gene in Colorectal Adenocarcinoma*

作　　者：王延雷[1] 张建立[1] 高军[1] 王政坤[1] 邱志刚[1] 谭晓杰[1] 

机构地区：[1]青岛大学医学院附属医院普外科,山东青岛266071

出　　处：《现代生物医学进展》2013年第15期2951-2954,共4页Progress in Modern Biomedicine

基　　金：山东省自然科学基金项目(Y2007C030)

摘　　要：目的:探讨人巨细胞病毒(human cytomegalovirus,HCMV)感染在结直肠癌发生、发展过程中的作用及其可能的作用机制。方法:选择84例结直肠腺癌患者的腺癌组织及自身癌旁正常组织,应用RT-PCR技术检测组织中即刻早期基因2(immediate-earlygene 2,IE2)mRNA的阳性率来反映HCMV的感染情况,免疫组化技术检测凋亡相关基因C-Jun的表达情况。结果:HCMV IE2基因在结直肠腺癌组织中的阳性率高于癌旁组织(P<0.01);C-Jun基因在结直肠腺癌组织中表达的阳性率高于癌旁组织(P<0.01);HCMV感染与C-Jun基因在结直肠腺癌组织内的表达存在关联性。结论:HCMV感染在结直肠癌的发生、发展中具有一定作用,其作用机制可能与C-Jun基因的表达有关。Objective： To investigate the effect of human cytomegalovirus（HCMV） infection on the development of colorectal adenocarcinoma and its possible mechanisms.Methods： RT-PCR was used to detect the expression of immediate-early gene 2（IE2） mRNA to reflect HCMV infection while the expression of C-Jun in 84 colorectal adenocarcinoma tissues and adjacent tissues was evaluated with immunohistochemical straining.Results： The positive rate of HCMV IE2 in colorectal adenocarcinoma tissues was higher than that in the adjacent tissues（P0.01）;The positive rate of C-Jun in colorectal adenocarcinoma tissues was also higher than that in the adjacent tissues（P0.01）;The expression of C-Jun gene in colorectal adenocarcinoma tissues corelated with HCMV infection（P0.05）.Conclusion： HCMV infection may play an important role in the development and progression of colorectal adenocarcinoma,and its mechanisms may be related with the expression of C-Jun.

关 键 词：结直肠肿瘤 人巨细胞病毒 C-JUN基因 

分 类 号：R735[医药卫生—肿瘤]