脑深部电刺激自身给药大鼠伏隔核区△FosB的表达  

△FosB Expression after Deep Brain Stimulation of the Nucleus Accumbens in the Rat Morphine Self-Administration Model

作　　者：屈晓鹏[1] 李江[1] 崔颖[1] 冯富强[1] 高国栋[1] 

机构地区：[1]第四军医大学唐都医院神经外科,陕西西安710038

出　　处：《现代生物医学进展》2013年第16期3052-3055,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81101022)

摘　　要：目的:对吗啡依赖大鼠实施双侧伏隔核脑深部电刺激(NAc-DBS),分析NAc区△FosB的表达变化,为NAc-DBS治疗药物依赖提供分子生物学证据。方法:18只大鼠随机分为三组,包括DBS组(n=6,实施颈静脉插管和电极植入手术,吗啡给药,DBS),Sham组(n=6,实施颈静脉插管和电极植入手术,吗啡给药),Control组(n=6,实施颈静脉插管和电极植入手术,给予生理盐水),观察DBS组大鼠在高频电刺激期(160 Hz,1 h/d,7 d)的觅药行为变化,然后进行取脑,切片,免疫组化染色,观察伏隔核区△FosB的表达。结果:成瘾大鼠在高频电刺激期,觅药行为明显减少;免疫组化染色后观察到DBS组大鼠NAc区△FosB的表达相对于Sham和Control组明显减少。结论:双侧NAc-DBS抑制吗啡成瘾大鼠的觅药行为以及NAc区△FosB的表达,证实△FosB可能是慢性成瘾转换机制的关键分子的观点。Objective： To take the implementation of the bilateral NAc-DBS surgery on morphine-addiction rats.Then we analyzed the change of △FosB expression in NAc which providing molecular evidence for the NAc-DBS in the treatment of drug dependence.Methods： 18 adult rats weighing 240-260 g were divided into three groups,which including the DBS group（n=6,administration of surgery,morphine and DBS）,the sham DBS group（n= 6,administration of surgery and morphine） and the control group（n= 6,administration of physiological saline）.Results： The bilateral NAc DBS attenuated morphine-induced reinstatement of drug seeking.The following immunohistochemistry measurements revealed that the behavioral consequences should be associated with a significant reduction in the expression of △FosB in the NAc.Conclusion： These findings have indicated that the NAc DBS could be an effective and safe therapeutic option for preventing relapse to morphine addiction.

关 键 词：自身给药 吗啡 △FosB 脑深部电刺激 伏隔核 

分 类 号：R749.64[医药卫生—神经病学与精神病学]