周期性张应力对大鼠髁突软骨细胞Ⅱ型胶原表达的影响  

Effect of Cyclic Tensile Stress on the Expression of Type Ⅱ Collagen in Rat Condyle Chondrocyte

作　　者：李朝阳[1] 李娟[2] 郑如松[2] 杜衍晓 杨美平[1] 杨杰[1] 

机构地区：[1]青岛大学医学院附属医院海阳分院口腔科,山东海阳265100 [2]青岛大学医学院附属医院海阳分院,山东海阳265100 [3]青岛市中心医院,山东青岛266042

出　　处：《现代生物医学进展》2013年第16期3056-3059,共4页Progress in Modern Biomedicine

摘　　要：目的:在成功构建髁突软骨细胞体外培养-力学刺激模型的基础上,探讨周期性张应力对髁突软骨细胞主要细胞外基质(Ⅱ型胶原)合成的影响。方法:本研究采用FX-5000T应力加载系统对体外培养的第3代大鼠髁突软骨细胞分别施加1 h、6 h、12 h和24 h的周期性张应力,应力刺激强度为10℅1 HZ。加力完成后即刻收集加力细胞,提取细胞总RNA反转录成cDNA,应用RT-PCR技术检测髁突软骨细胞主要细胞外基质Ⅱ型胶原(type-Ⅱcollagen,Col-Ⅱ)mRNA的表达变化情况。结果:与对照组(0 h组)相比,加力1 h时Col-Ⅱ的表达增加,但无统计学意义;加力6 h时Col-Ⅱ表达显著增加(P<0.05);加力12 h时Col-Ⅱ表达开始下降;当加力至24 h时表达量显著降低(P<0.05)。结论:周期性张应力可以影响髁突软骨细胞主要细胞外基质的合成,在一定范围内随加力时间的延长基质合成逐渐增强;进一步延长加力时间,基质的合成受到明显抑制。Objective： To study the effect of cyclic tensile stress on condyle chondrocyte main extracellular matrix based on successful construction of in vitro mechanical stimulation models.Methods： The cyclic tensile stress was exposed to the third passage condyle chondrocyte for 1,6,12 and 24 hours respectively by using a Flexcell Strain Unit-5000T（10℅surface elongation,6 cycles/min）.After mechanical loading,total RNA was extracted from the cells harvested from Six-well BioFlex flexible cell Petri Dish,and RT-PCR was performed to quantify mRNA levels for type Ⅱ collagen.Results： Compared with the control group（0h group）,type Ⅱ collagen mRNA expression was increased after 1 h loading,but there were no significant differences between loading and control group;after 6h cyclic tensile stress,the expression of Col-II increased significantly（P0.05）;after 12 and 24 h of loading,the expression for this cartilage-specific matrix protein reduced remarkably（P0.05）.Conclusion： The result illustrated that a cyclical tensile stress stimuli can affect the main condylar cartilage extracellular matrix synthesis,Col-Ⅱ can be promoted during early loading,with extended matrix synthesis increased continue,afterwards,further extend the matrix synthesis will be inhibited.

关 键 词：功能矫形 张应力 髁状突软骨 Ⅱ型胶原 

分 类 号：Q95-3[生物学—动物学] R783.5[医药卫生—口腔医学]