RP-HPLC测定蒜氨酸原料药中的有关物质  

Determination of the Related Substance in Alliin by RP-HPLC

作　　者：黄洪勇[1] 崔利娜[2] 唐辉[2] 黄瑞廷[2] 

机构地区：[1]新乡医学院第二附属医院,河南新乡453002 [2]石河子大学药学院新疆特种植物药资源省部共建教育部重点实验室,新疆石河子832002

出　　处：《中国实验方剂学杂志》2013年第12期101-104,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：新疆维吾尔自治区高技术研究与发展计划项目(200511112)

摘　　要：目的:建立测定蒜氨酸中试原料药中有关物质的方法。方法:采用RP-HPLC,DIKMA DiamonsilTMC18色谱柱(4.6mm×250 mm,5μm),以水为流动相,流速0.8 mL.min-1,柱温25℃,检测波长220 nm。结果:蒜氨酸与相邻杂质峰能完全分离,蒜氨酸在0.049 6～1.008 6μg进样量与峰面积呈良好线性关系(r=0.999 6),最低检测限为4 ng,精密度RSD 0.43%,平均回收率为99.22%(n=9)。结论:该方法简便、准确、专属性好、灵敏度高,可用于控制蒜氨酸中试原料药的质量。Objective： To establish a method to determine the related substances of alliin.Method： RPHPLC was adopted for the determination of the related substances of alliin.The chromatographic procedure was carried out with DIKMA DiamonsilTMC18（4.6 mm ×250 mm,5 μm） as an analytical column and water as mobile phase at a flow rate of 0.8 mL.min-1 at 25 ℃.The detection wavelength was set at 220 nm.Result： There was a good linear relationship within the ranger of 0.049 6-1.008 6 μg（r = 0.999 6）,the limit of detection for alliin was 4 ng.The precision was 0.43%,the average recovery was 99.22%（n = 9）.Conclusion： The method is simple,accurate,specific and sensitiveness for quality control of alliin.

关 键 词：蒜氨酸 有关物质 高效液相色谱 

分 类 号：R284.1[医药卫生—中药学]