空肠弯曲杆菌临床株生物膜模型的建立及影响因素的研究  

Establishment of Biofilm Model in Vitro of Clinical C.jejuni isolation and Studies of its Influence Factors

作　　者：秦晓庆[1] 赵树臣[1] 侯振中[1] 

机构地区：[1]东北农业大学动物医学学院,黑龙江哈尔滨150030

出　　处：《中国兽医杂志》2013年第5期6-8,I0001,共4页Chinese Journal of Veterinary Medicine

摘　　要：本研究选取空肠弯曲杆菌(C.jejuni)临床分离株作为研究对象,建立其生物膜体外模型,通过PCR、扫描电镜以及正交试验等方法,研究其相关特性及影响因素,为临床防治该病原菌并研究其耐药性的消除方面提供一定的理论依据。C. jejuni is a worldwide prevalent pathogen of zoonotic disease, was very hypercritical to the living conditions. In recent years, some scholars had found that, the bacteria could form a biofilm to resist the adverse external environmental impact under certain conditions, this was also the important rea- son for the bacteria to survive harsh environment and drug resistance. We chose Campylobacter jejuni iso- lated from clinical samples as the research object in this study, built the biofilm model in vitro, established by a series of methods, such as PCR, scanning electron microscope and the orthogonal experiment, to study the characteristics and influence factors of the bioflm, looking forward to getting some useful results for clinical prevention and treatment and elimination of the drug resistance.

关 键 词：C JEJUNI 生物膜 PCR 扫描电镜 正交试验 影响因素 

分 类 号：R378[医药卫生—病原生物学]