利用UV和EMS诱变选育高产油微藻藻株的研究  

Isolation and characterization of high-oil microalgal mutant strains using UV and EMS treatments

作　　者：沈丹丹[1,2] 杨芳芳[1,2] 万凌琳[1,2] 李爱芬[1,2] 张成武[1,2] 

机构地区：[1]暨南大学生命科学技术学院,广州510632 [2]暨南大学水生生物研究所,广州510632

出　　处：《中国油脂》2013年第6期60-64,共5页China Oils and Fats

基　　金：国家"863"项目(2009AA06440;2013AA065805);国家"973"项目(2011CB2009001);国家自然科学基金项目(31170337);广东省教育部产学研究结合项目(2010A090200008);珠海市科技重点项目(PC20081008);广东省低碳发展专项资金(2011-051)

摘　　要：分别采用不同剂量的紫外线(UV)和甲基磺酸乙酯(EMS)诱变2种真眼点藻(波氏真眼点藻(Eustigmatos polyphem)和魏氏真眼点藻(Eustigmatos vischeri)),以氯霉素、红霉素、硫酸新霉素、硫酸卡那霉素为抗性筛选剂,利用抗生素最小致死浓度法筛选出抗性突变株。使用改良的BG-11培养基对反复筛选所获得的抗性突变株进行固、液培养,并以生物量干重、干藻粉的总脂含量、单位体积总脂含量、单位体积总脂产率为评价指标对抗性突变株的优劣进行评价。结果获得2株具稳定遗传特性、生长速度快、单位体积总脂产率高和具生产潜力的优良突变株。Different doses of ultraviolet ray （UV） and ethyl methyl sulphonate （EMS） were used as mu- tagen to treat two eustigmatophyte microalgaes （Eustigmatos polyphem, Eustigmatos vischeri） respectively. According to the minimum antibiotics lethal concentration approach, chloromycetin, erythromycin, neomy- cin sulfate and kanamycin sulfate were chosen as resistance agent to screen mutant strains. The different mutant strains were cultured in modified solid and liquid BG - 11 mediums for assessing their growth and lipid accumulation. Dry biomass weight, total lipid content, volumetric total lipid content and volumetric total lipid productivity were employed to evaluate the properties of the selecting mutant strains. Finally, two excellent mutant strains with fast growth, high volumetric total lipid productivity, stable genetic char- acteristics and potentially commercial development value were obtained.

关 键 词：紫外线 甲基磺酸乙酯 突变藻株 总脂含量 单位体积总脂含量 单位体积总脂产率 

分 类 号：S968.49[农业科学—水产养殖]