漆酶/TBHP催化黄麻接枝丙烯酰胺的研究  

Study on graft copolymer of jute and AM initiated by laccase/TBHP

作　　者：周帅红[1] 王强[1] 范雪荣[1] 张亚[1] 袁久刚[1] 王平[1] 崔莉[1] 

机构地区：[1]江南大学生态纺织教育部重点实验室,江苏无锡214122

出　　处：《生物学杂志》2013年第3期89-93,共5页Journal of Biology

基　　金：国家自然科学基金项目(51173071);教育部长江学者和创新团队发展计划(IRT1135);教育部高等学校博士学科点专项科研基金资助项目(20110093110003);江苏省自然科学基金项目(BK2011157);江苏省"六大人才高峰"资助项目(2011-XCL-002);江苏高校优势学科建设工程资助项目(〔苏政办发2011〕137号)

摘　　要：以富含木质素的黄麻为原料,在漆酶/TBHP介质体系下,通过漆酶催化木质素氧化引发丙烯酰胺在黄麻表面接枝共聚。利用凯氏定氮法测定了接枝前后黄麻氮含量变化,傅里叶红外光谱分析了接枝产物结构;考察了反应温度、pH值、单体用量和漆酶浓度对接枝率和接枝效率的影响。结果表明:未接枝的黄麻含氮量为1.16 mg/g,经漆酶/TBHP体系催化接枝并去除均聚物和未反应单体后的黄麻含氮量达到5.19 mg/g,在接枝黄麻的红外光谱图上检测到了酰胺的红外特征吸收峰,说明黄麻表面确实接枝了聚丙烯酰胺;温度50℃、pH值5、AM/黄麻2∶1、漆酶浓度2 U/mL时接枝效果最好,此时接枝率达到2.87%,接枝效率为1.43%。Abstract： Acrylamide（AM） was grafted to the surface of jute fabric which is rich in lignin using laccase/TBHP as the catalyst. The Kjeldahl nitrogen determination method was used to measure nitrogen content of jute graft-copolymer, and the structure of the copolymer was analyzed by FT-IR. The effects of graft polymerization conditions including reaction temperature, pH value and the dosages of monomers and laceases on grafting percentage and grafting efficiency were assessed. The results indicated that the nitrogen contents of ungrafted jute and jute graft-copolymer were 1.16 mg/g and 5. 19 mg/g, respectively. Amide absorption peak detected on the infrared spectrum of grated jute revealed the existence of jute graft-copolymer. The optimal conditions were obtained as follows： reaction temper- ature 50℃, pH value 5, consumption rate of AM to jute 2 ： 1, the dosage of laccase 2U/mL. Under these conditions, the grafting per- centage of jute graft-copolymer was 2.87% and grafting efficiency reached 1.43%.

关 键 词：黄麻 木质素 丙烯酰胺 漆酶 接枝共聚 

分 类 号：TS193.1[轻工技术与工程—纺织化学与染整工程]