高效液相色谱法测定西兰花籽提取物中萝卜硫苷含量  

Determination of gulcoraphanin in broccoli seeds extracts by HPLC

作　　者：石林娟[1] 潘见[1] 谢慧明[1] 王园[1] 

机构地区：[1]农产品生物化工教育部工程研究中心,合肥工业大学,合肥230009

出　　处：《食品科技》2013年第6期231-236,共6页Food Science and Technology

基　　金：企业委托项目

摘　　要：目的:建立萝卜硫苷(4-甲基亚磺酰丁基硫代葡萄糖苷)的定量测定方法。方法:西兰花籽中的萝卜硫苷经溶剂提取,离子交换柱纯化,采用高效液相色谱法(HPLC)进行测定。色谱柱为Global Chromatography SP-120-5-C18-AP(5μm,4.6mm×200mm),以甲醇:水=1:9(含0.1%甲酸,pH4.0)为流动相,检测波长为229nm,流速为1.0mL/min,柱温为室温。结果:在该色谱条件下萝卜硫苷与其他物质得到了有效的分离,且萝卜硫苷在0.14～2mg/mL范围内与峰面积有良好的线性关系,R=0.9996,检测限为0.08mg/mL,萝卜硫苷的加样回收率为99.45%,RSD为0.77%。结论:建立的方法重复性好、精密度高、回收率较好,可作为西兰花籽提取物中萝卜硫苷的定量测定方法。Objective： To establish the method quantitative determination of gulcoraphanin （4-Methylsufinylbutyl glucosionlate）. Methods： The gulcoraphanin was extracted from broccoli seeds by solvent, purified by ion exchange column, then detected by high performance liquid chromatogram （HPLC）. The analysis was performed on Global Chromatography SP-120-5-C18-AP column with the methanol- water=l：9 （including 0.1% formic acid, pH4.0） as mobile phase, the detecting wavelength was 229 nm and the flow rate was 1.0 mL/min, the column temperature is room temperature. Results： The results show that the gulcoraphanin with other material got effective separation on this detecting conditions, and the gulcoraphanin had good linear relationship with the range of 0.14-2 mg/mL, R=0.9996, the detection limit was 0.08 mg/mL. The recoveries of gulcoraphanin were 99.45%. Conclusion： The method is good on reproducibility, high in precision, recovery is better, which can be used as the method of quantitative determination of gulcoraphanin in broccoli seeds extracts.

关 键 词：西兰花籽提取物 萝卜硫苷 定量测定 高效液相色谱法 

分 类 号：TS255.7[轻工技术与工程—农产品加工及贮藏工程]