漆树酸和阿霉素联合应用抗肝癌的协同作用  

A synergistic effect of anacardic acid combined with doxorubicin on liver cancer

作　　者：董晓先[1] 周萍[1] 黄洪标[1] 

机构地区：[1]广州医学院病理生理学教研室,510182

出　　处：《国际医药卫生导报》2013年第11期1562-1565,共4页International Medicine and Health Guidance News

基　　金：广州医学院博士启动基金资助项目（201IC24）;广东省高校优秀青年创新人才培养计划项目（2012LYM一0109）

摘　　要：目的评价漆树酸与阿霉素联合应用对人肝癌HepG2细胞是否具有协同作用。方法人月十癌HepG2细胞经漆树酸和（或）阿霉素处理后，MTS法检测细胞的增殖情况，Westernblot方法检测Caspase-3，8，9和PARP凋亡相关蛋白的变化。结果漆树酸和阿霉素联合应用能够明显增强抑制HepG2细胞增殖，与单药组相比具有明显的协同作用。漆树酸和阿霉素联合应用可以活化Caspase-3、Caspase-8、Caspase-9，引起PARP的切割。结论漆树酸和阿霉素联合应用具有明显的协同作用，能够增强抑制HepG2细胞的增殖，两药联合作用的机制可能与诱导Caspase系统活化相关。Objective To explore whether anacardic acid combined with doxorubicin has a synergistic effect on human HepG2 cells. Methods After treatment with anacardic acid and／or doxorubicin, the proliferation of＇ HepG2 ceils was detected by MTS, and the changes of Caspase-3, -8, and -9 and PARP were examined by Western blot. Results As compared with treatment with anacardic acid or doxornbicin alone, anacardic acid plus doxorubicin significantly enhanced the inhibition of HepG2 proliferation, with a marked synergistic effect; and it activated Caspase-3, -8, and -9 and induced the cutting of PARP. Conclusions Anacardie acid combined with doxorubicin has a synergistic effect on the inhibition of HepG2 proliferation, whose mechanism may be related to the induction of activation of the Caspasesystem.

关 键 词：漆树酸 阿霉素 肝癌 协同 

分 类 号：R735.7[医药卫生—肿瘤]