山蕨菜组织培养的研究  

The Experiment of Tissue Culture on Pteridium Aquilium

作　　者：赵彦芳[1] 娄和林[1] 雷秀云 关崇新 张世明 高光辉 

机构地区：[1]辽宁大学生物系,沈阳110036 [2]岫岩县人民政府,岫岩114300

出　　处：《辽宁大学学报（自然科学版）》2000年第1期76-79,共4页Journal of Liaoning University：Natural Sciences Edition

摘　　要：以蕨菜(pteridiumaquiliumL.)幼嫩根状茎先端为外植体,经灭菌、接种于附加有多种激素、磷酸二氢钠、活性炭等物质的1/2MS培养基上,在一定条件下,接种近三个月时,外植体上分化形成了聚集成团的不定芽丛,反复切割、转移培养于继代培养基上,分化势能不减.在附加有GA的培养基上,芽轴伸展并长出幼叶(羽状分裂叶).转移至生根培养基上20余天时,不定根群长出,但移栽成活率较低(仅40%左右).Treat the young rhizome's tip of pteridium aquilinum as the explants, after sterilized, inoculated to the 1/2 MS medium which contains NaH 2PO 4, active carbon and some hormones. When cultured almost for three months, the tip of pteridium aquilinum dfferentiated, on the top of the explant a group of green small adventitious buds were evolved. Cut off the adventitious buds and transplant onto new medium repeatedly, the differentiation potential of bud keeps still. On the medium with GA the stem of the adventitious buds stretched and some little leafs were grown. Transplant the shoot without root onto 1/2 MS medium only with NAA, twenty days after, the roots were grown. Take 1 2 days for exercise, the regenerate plantlet maybe plant in soil, the survival rate were under 40%.

关 键 词：山蕨菜 根状茎 再生植株 山野菜 

分 类 号：Q949.36[生物学—植物学] S647[农业科学—蔬菜学]