FAT10过表达促进人乳头状甲状腺癌细胞抗凋亡及集落形成能力的研究  

Effect of FAT10 Over-expression on Anti-apoptosis and Colony-forming Capacity of Papillary Thyroid Cancer Cells

作　　者：高芸[1] 商雪莹[1] 吕男男[1] 滕卫平[1] 单忠艳[1,2] 

机构地区：[1]中国医科大学附属第一医院内分泌研究所,辽宁沈阳110001 [2]中国医科大学附属第一医院内分泌科

出　　处：《实用预防医学》2013年第6期647-649,共3页Practical Preventive Medicine

基　　金：国家自然科学基金项目(No.81102471)

摘　　要：目的探讨FAT10过表达对人乳头状甲状腺癌细胞抗凋亡及集落形成能力的影响。方法采用脂质体法将FAT10质粒导入TPC-1细胞,建立FAT10过表达的人乳头状甲状腺癌细胞,用Western blot检测细胞中的FAT10表达水平,用染色体核型分析FAT10过表达而导致的非整倍体现象,Annexin V凋亡试剂盒检测FAT10过表达对TPC-1抗凋亡能力的影响。通过集落形成实验观察其集落形成能力的变化。结果 Western blot结果显示FAT10过表达组细胞FAT10蛋白水平明显高于对照组,FAT10过表达组出现非整倍体形象,并且抗喜树碱诱导凋亡的能力和集落形成能力显著高于对照组。结论 FAT10过表达可以促进人乳头状甲状腺癌细胞抗凋亡及集落形成能力。Objective To study the effect of FAT10 over-expression on anti-apoptosis and colony-forming capacity of papillary thyroid cancer cell line.Methods Recombinant plasmid pcDNA3-FAT10 was transfected into papillary thyroid cancer cell line TPC-1 by lipofectamine 2000.The efficiency of FAT10 over-expression was assessed by Western blot.Cytogenetic analysis was performed to analyze the chromosome numbers.The effects of FAT10 over-expression on anti-apoptosis and colony-forming capacity were determined by Annexin-V and colony formation assay,respectively.Results Western blot results demonstrated that the protein level of FAT10 was significantly increased in the FAT10 over-expression group when compared with that in the negative control and parental group.FAT10 over-expression induced abnormal chromosome karyotype in TPC-1 cells and significantly promoted anti-apoptosis and colony-forming capacity.Conclusions Over-expression of FAT10 can promote anti-apoptosis and colony-forming capacity in TPC-1 cells.

关 键 词：FAT10 乳头状甲状腺癌 抗凋亡 集落形成 

分 类 号：R730.231[医药卫生—肿瘤]